Transcriptome analysis of peach fruit under 1-MCP treatment provides insights into regulation network in melting peach softening  

作　　者：Jianpu Qian Yingjie Zhao Yanna Shi Kunsong Chen 钱健璞;赵颖婕;石艳娜;陈昆松(College of Agriculture and Biotechnology,Zhejiang University,Zijingang Campus,Hangzhou,China;Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology,Zhejiang University,Zijingang Campus,Hangzhou,China;State Agriculture Ministry Laboratory of Horticultural Plant Growth,Development and Quality Improvement,Zhejiang University,Zijingang Campus,Hangzhou,China)

机构地区：[1]College of Agriculture and Biotechnology,Zhejiang University,Zijingang Campus,Hangzhou,China [2]Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology,Zhejiang University,Zijingang Campus,Hangzhou,China [3]State Agriculture Ministry Laboratory of Horticultural Plant Growth,Development and Quality Improvement,Zhejiang University,Zijingang Campus,Hangzhou,China

出　　处：《Food Quality and Safety》2022年第4期455-465,共11页食品品质与安全研究（英文版）

基　　金：supported by the 111 Project(B17039);the Fundamental Research Funds for the Central Universities(226-2022-00039),China.

摘　　要：Peach(Prunus persica L.)displays distinguish texture phenotype during postharvest,which could be classified into three types,including melting flesh(MF),non-melting flesh,and stony-hard.MF peach would soften rapidly with an outbreak of ethylene production,which causes huge wastage during fruit transportation and storage.1-Methylcyclopropene(1-MCP)was used to alleviate fruit softening.In this study,we performed RNA-sequencing on two MF peach cultivars(‘YuLu’and‘Yanhong’)after 1-MCP treatment to identify the candidate genes participating in peach fruit softening.A total of 167 genes were identified by weighted gene coexpression network analysis and correlation analysis that could respond to 1-MCP treatment and might be related to softening.Among them,5 auxin-related genes including 2 indole-3-acetic acid,1 auxin response transcription factor and 2 small auxin-up RNAs,and 4 cell wall modifying genes(PpPG1,PpPG2,PpPG24 and PpPMEI)were characterized as key genes participating in MF peach softening.Furthermore,2 transcription factors,which belong to HD-ZIP and MYB,were predicted as candidates regulating softening process by constructing a transcriptional network of these 4 cell wall modifying genes combined with expression pattern analysis,of which HD-ZIP could trans-activate the promoter of PpPG1.

关 键 词：PEACH SOFTENING HORMONE cell wall transcription factor 

分 类 号：S66[农业科学—果树学]