Rapid detection of fluoroquinolone residues in aquatic products based on a gold-labeled microwell immunochromatographic assay  

作　　者：Jiaxu Xiao Hongfei Yang Liangni Qin Jixiang Liang Long Li Xiaohui Fan Dapeng Peng 

机构地区：[1]National Reference Laboratory of Veterinary Drug Residues/Key Laboratory of Ministry of Agriculture for the Detection of Veterinary Drug Residues in Foods,Huazhong Agricultural University,Wuhan,China [2]Wuhan Shangcheng Biotechnology Co.,Ltd.,Wuhan,China [3]Shenzhen Institute of Nutrition and Health,Huazhong Agricultural University,Shenzhen,China [4]Shenzhen Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,China [5]Genome Analysis Laboratory of the Ministry of Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,China

出　　处：《Food Quality and Safety》2022年第4期597-607,共11页食品品质与安全研究（英文版）

基　　金：funded by the Key Research and Development Program of Hubei Province(CN)(No.2020BBB079);the Fundamental Research Funds for the Central Universities(2662022DKPY007);the HZAU-AGIS Cooperation Fund(SZYJY2022024),China.

摘　　要：Objectives:Fluoroquinolones(FQs)are widely used in aquaculture,and their residues have caused many problems threatening human health.Here,this study aims to develop a colloidal gold immunochromatographic strip based on gold-labeled microwells to screen the residues of FQs on site.Materials and methods:The Protein A Magarose Beads affinity chromatography method was adopted to purify the ascites against FQs.By using a strategy of heterologous coating antigen,different coating antigens are applied to detect FQs.The gold-labeled microwell immunochromatographic assay was used to improve the sensitivity of the test strip by the advanced reaction of antigen and antibody.Results:The antibodies were verified to be of high purity up to 99%,and the titer reached 1:1024000.The combination(enoxacin-OVA and the antibody)detected the 4 banned FQs(pefloxacin,PEF;norfloxacin,NOR;lomefloxacin,LOM;ofloxacin,OFL)with the 50%inhibiting concentration(IC50)values ranging from 1.3 to 2.1 ng/mL and cross-reactions ranging from 67.3%to 106.1%.The analysis of spiked crucian carp,silver carp,grass carp,and shrimp samples showed that the limit of detection for PEF,NOR,LOM,and OFL was 4μg/kg.A comparative study with liquid chromatography tandem mass spectrometry(LC-MS/MS)demonstrated that the assay provided an effective screening tool for the rapid detection of FQs residues.Conclusions:The results indicated that the test strip can realize full coverage recognition of the 4 banned FQs and has good accuracy,specifi-city,reproducibility,and stability;therefore,they are more suitable for rapid detection of FQs in aquatic products.

关 键 词：FLUOROQUINOLONES colloidal gold MICROWELL PURIFICATION RESIDUES 

分 类 号：Q95[生物学—动物学]