Bacterial pathogens and factors associated with Salmonella contamination in hybrid red tilapia(Oreochromis spp.)cultivated in a cage culture system  

作　　者：Varangkana Thaotumpitak Jarukorn Sripradite Edward R.Atwill Surapun Tepaamorndech Saharuetai Jeamsripong 

机构地区：[1]Research Unit in Microbial Food Safety and Antimicrobial Resistance,Department of Veterinary Public Health,Faculty of Veterinary Science,Chulalongkorn University,Bangkok,Thailand [2]Department of Social and Applied Science,College of Industrial Technology,King Mongkut's University of Technology North Bangkok,Bangkok,Thailand [3]Department of Population Health and Reproduction,School of Veterinary Medicine,University of California,Davis,Davis,CA,USA [4]Functional Ingredients and Food Innovation Research Group,National Center for Genetic Engineering and Biotechnology(BIOTEC),Pathum Thani,Thailand [5]Department of Microbiology,Faculty of Medicine,Chulalongkorn University,King Chulalongkorn Memorial Hospital,Bangkok,Thailanc

出　　处：《Food Quality and Safety》2022年第3期404-415,共12页食品品质与安全研究（英文版）

基　　金：supported by the University of California,Davis,USA (A19-4577-S001);partially funded by the Faculty of Veterinary Science,Chulalongkorn University,Thailand (RG17/2559);the 90th Anniversary of Chulalongkorn University fund (Ratchadaphiseksomphot Endowment Fund);supported by the National Research Council of Thailand (NRCT5-RGJ63001-017).

摘　　要：Microbial food safety in cultured tilapia remains a challenge to public health worldwide,due in part to intensive aquaculture leading to poor water quality and high organic matter deposition.This study aimed to determine the prevalence of indicator and potential pathogenic bacteria in hybrid red tilapia(Oreochromis spp.)and their cultivation water and to identify environmental parameters and other bacterial contaminants associated with Salmonella contamination.A total of 120 fish were sampled,which were partitioned into fish carcasses(n=120),muscle(n=120),intestine(n=120),liverandkidney(n=120),and cultivationwater(n=120)from three commercial farms in westernThailand from October2019 to November 2020.The prevalence of fecal coliforms and Escherichia coli(E.col)in these 600 samples was 74.8%and 56.7%,respectively.The prevalence of Salmonella,Vibrio cholerae(V.cholerae),Aeromonas hydrophila,and Vibrio vulnificus(V.vulnificus)was 23.0%,17.5%,2.5%,and 1.7%,respectively.None of the samples tested positive for Streptococcus agalactiae.Cultivation water exhibited a high prevalence for Salmonella(58.3%).Among fish samples,Salmonella had the highest prevalence at 14.1%,which was mainly from fish intestine.There was a significant association of Salmonella with the presence of fecal coliforms,E.coli,V.cholerae,and V.vulnificus.The predominant serovars of Salmonella included Saintpaul,Neukoelln,Escanaba,and Papuana.Grazing ducks that were raised in proximity to these cultured tilapia shared the same isolates of Salmonella based on the similarity of their rep-PCR DNA fingerprints,suggesting that ducks may function as either a biological reservoir for tilapia or at minimum participate in the environmental replication of this strain of Salmonella.Taken together,the results suggest that the environment used for tilapia aquaculture may be contaminated with pathogenic bacteria;therefore,food safety precautions are needed during processing,transportation,cooking,and consumption.

关 键 词：Aeromonas spp grazing duck Salmonella spp TILAPIA Vibrio spp 

分 类 号：R155.5[医药卫生—营养与食品卫生学]