Inhibition of citral nanoemulsion to growth,spoilage ability and Al-2/luxS quorum sensing system of Shewanella putrefaciens CN-32:a study on bacteriostasis from in vitro culture and gene expression analysis  

作　　者：Zhiheng Hu Yaoxian Chin Jiayin Huang Jiaying Zhou Gaoshang Li Yaqin Hu Chunhong Yuan Jianchu Chen 

机构地区：[1]College of Biosystems Engineering and Food Science,National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment,Zhejiang Key Laboratory for Agro-Food Processing,Integrated Research Base of Southern Fruit and Vegetable Preservation Technology,Fuli Institute of Food Science,Zhejiang University,Hangzhou,China [2]College of Food Science and Engineering,Hainan Tropical Ocean University,Yazhou Bay Innovation Institute,Marine Food Engineering Technology Research Center of Hainan Province,Collaborative Innovation Center of Marine Food Deep Processing,Sanya,China [3]Department of Food Production and Environmental Management,Faculty of Agriculture,Iwate University,Ueda4-3-5,Morioka,Japan

出　　处：《Food Quality and Safety》2022年第3期435-445,共11页食品品质与安全研究（英文版）

基　　金：supported by the Hainan Provincial Natural Science Foundation of China (321CXTD1012);the National Natural Science Foundation of China (NSFC31871868);the Scientific Research Foundation of Hainan Tropical Ocean University (RHDRC202117),China.

摘　　要：Objectives:The bacteriostatic effects of a citral nanoemulsion against Shewanella putrefaciens CN-32(SHP CN-32)were investigated using in vitro culture and gene expression analysis,forbuilding a potential application in spoilage microorganism control and aquatic products quality maintenance.Materials and Methods:SHP CN-32 was treated by prepared citral nanoemulsion when the minimal inhibitory concentration(MIC)was verified.The growth curve,membrane integrity,scanning electron microscope(SEM)observation,biofilm formation and quorum sensing(QS)signaling molecule Al-2 content were evaluated in different MIC treatment groups(0 to 1.00 MIC).The gene expression status of SHP CN-32 in O and 0.50 MIC groups were compared using transcriptome sequencing and quantitative polymerase chain reaction(PCR).Results:The in vitro culture revealed that the citral nanoemulsion could inhibit the growth of SHP CN-32 with MIC of approximately 200μg/mL.Images of membrane integrity.SEM and biofilm formation suggested significant biological structure damage in bacteria after treatment.Meanwhile,the Qs signaling molecule Al-2 content showed a decline with increasing treatment concentration.Transcriptome sequencing and quantitative PCR revealed that the majority genes related diversified functional metabolic pathways of SHP CN-32 were downregulated at varying degree.Conclusion:A significant bacteriostasis of citral nanoemulsion against SHP CN-32 was verified via the results of growth inhibition,structural destruction,signal molecular decrease and gene expression downregulation of strains.These synergies significantly affect the characteristic expression of SHP CN-32,revealing the application potential as bacteriostat,QS inhibitor and preservative in aquatic products.

关 键 词：Shewanella putrefaciens CITRAL quorum sensing system gene expression analysis 

分 类 号：R155.5[医药卫生—营养与食品卫生学]