紫茎泽兰发酵前后挥发油成分变化及其抑菌活性  

Study of Volatile Oil Composition and Antibacterial Activity of Eupatorium Adenophorum Before and After Fermentation

作　　者：刘洋[1] 胡世俊 张东华[1] 皋玉功 闫晓慧[1] LIU Yang;HU Shi-jun;ZHANG Dong-hua;GAO Yu-gong;YAN Xiao-hui(Key Laboratory of Forest Disaster Warning and Control in Yunnan Province,College of Biodiversity Conservation,Southwest Forestry University,Kunming 650224,China;College of Forestry,Southwest Forestry University,Kunming 650224,China;Eupatorium Adenophorum Technology(Yunnan)Co.,Kunming 650233,China)

机构地区：[1]西南林业大学生物多样性保护学院云南省森林灾害预警与控制重点实验室,云南昆明650224 [2]西南林业大学林学院,云南昆明650224 [3]紫茎泽兰中医农业科技云南有限公司,云南昆明650233

出　　处：《化学试剂》2023年第4期74-80,共7页Chemical Reagents

基　　金：国家自然科学基金项目(32160378);云南省应用基础研究重点项目(2019FA011);云南省高层次人才项目(YNWR-QNBJ-2018-300)。

摘　　要：分析紫茎泽兰微生物发酵前、后挥发油成分及其抑菌作用,为利用紫茎泽兰开发发酵饲料提供依据。利用GC-MS从紫茎泽兰发酵前、后挥发油中分别鉴定出40、43个化合物,含氧单萜类成分含量发酵后明显降低,从11.58%降低到7.12%,倍半萜烯类成分及含氧倍半萜烯类成分发酵后含量有所升高,分别由发酵前的28.9%、41.89%上升为发酵后的34.72%和46.32%。发酵后挥发油中柠檬烯、石竹烯等抑菌活性成分含量有所升高;抑菌活性结果表明发酵后挥发油对粪肠球菌的抑菌活性较好,抑菌圈直径达到16.40 mm,且发酵后挥发油对铜绿假单胞菌、大肠杆菌、粪肠球菌、沙门氏菌的抑菌圈直径都要高于发酵前;发酵前、后挥发油的MIC、MBC值范围为2.5~10.0μL/mL。本研究为资源化利用紫茎泽兰提供了一定的科学依据。The composition of volatile oil and its antibacterial effects before and after microbial fermentation of E.adenophorum were analyzed,which provided a basis for developing fermented forage with E.adenophorum.40 and 43 compounds were identified by GC-MS from the pre-fermentation and post-fermentation volatile oils,the content of the oxygenated monoterpenes decreased significantly after fermentation,from 11.58%to 7.12%,the contents of sesquiterpene and oxygen sesquiterpene increased after fermentation from 28.9%and 41.89%to 34.72%and 46.32%,respectively;and the concentration of limonene and sesquiterpene in the volatile oil increased after fermentation;the results of antibacterial activity showed that the inhibition activity of the volatile oil on E.faecalis was potent after fermentation,and the diameter of the inhibition circle was 16.40 mm After fermentation the diameter of inhibition circle of volatile oil on P.aeruginosa,E.coli,E.faecalis and S.enterica were larger than those before fermentation;the MIC and MBC values of volatile oil ranged from 2.5 to 10.0μL/mL.This study provided a scientific basis for the utilization of E.adenophorum.

关 键 词：紫茎泽兰 挥发油 微生物发酵 化学成分 抑菌活性 

分 类 号：R284.1[医药卫生—中药学]