基于响应面法优化谷氨酸棒杆菌发酵条件  

Optimization of Corynebacterium glutamate fermentation conditions based on response surface methodology

作　　者：上官玲玲 卢慧芳 张辉燕 李沛[2] 李库[2] 陈雄[1] 代俊[1] SHANGGUAN Lingling;LU Huifang;ZHANG Huiyan;LI Pei;LI Ku;CHEN Xiong;DAI Jun(College of Biological Engineering and Food,Provincial Ministry of Industrial Fermentation Collaborative Innovation Center,Key Laboratory of Fermentation Engineering Ministry of Education,Hubei University of Technology,Wuhan 430068,China;Hubei Key Laboratory of Yeast Function,Angela Yeast Co.,Ltd.,Yichang 443003,China)

机构地区：[1]湖北工业大学,发酵工程教育部重点实验室,工业发酵省部共建协同创新中心,生物工程与食品学院,湖北武汉430068 [2]安琪酵母股份有限公司,酵母功能湖北省重点实验室,湖北宜昌443003

出　　处：《中国酿造》2023年第3期202-208,共7页China Brewing

基　　金：国家自然科学基金项目(31871789,41876114);湖北省科技攻关计划(2020BGC010)。

摘　　要：该研究以谷氨酸棒杆菌(Corynebacterium glutamicum)P169为研究对象,以谷氨酸产量为主要评价指标,采用单因素试验和响应面法对其发酵条件进行优化,并进行摇瓶和20 L罐分批补料发酵验证。结果表明,谷氨酸棒杆菌P169产谷氨酸的最佳发酵条件为酵母粉41.0 g/L、葡萄糖27.0 g/L、尿素12.0 g/L和pH 7.0。在此优化条件下,谷氨酸产量达25.1 g/L,比优化前(16.5 g/L)提高了52.1%。以此为基料进行20 L罐分批补料发酵,谷氨酸产量达155 g/L,比优化前(142 g/L)提高了9.2%。该研究为提高谷氨酸棒杆菌谷氨酸产量提供了一种技术解决方案。Using Corynebacterium glutamicum P169 as research object,glutamic acid production as main evaluation index,the fermentation conditions were optimized by single-factor test and response surface methodology,and the fermentation conditions were verified by shake flask and 20 L tank batch replenishment.The results showed that the optimal fermentation conditions for glutamic acid production by C.glutamicum P169 were as follows:yeast powder 41.0 g/L,glucose 27.0 g/L,urea 12.0 g/L and pH 7.0.Under the optimal conditions,glutamic acid yield reached to 25.1 g/L,which was 52.1%higher than that before optimization(16.5 g/L).Based on the medium,20 L tank batch replenishment fermentation was conducted,and glutamic acid yield reached to 155 g/L,which was 9.2%higher than that before optimization(142 g/L).The study provided a technical solution for improving the glutamate yield of C.glutamate.

关 键 词：谷氨酸棒杆菌 谷氨酸 发酵条件 响应面法 

分 类 号：Q815[生物学—生物工程]