High Mannose-Specific Aptamers for Broad-Spectrum Virus Inhibition and Cancer Targeting  

作　　者：Wei Li Shuxin Xu Ying Li Jingran Chen Yanyan Ma Zhen Liu 

机构地区：[1]State Key Laboratory of Analytical Chemistry for Life Science,School of Chemistry and Chemical Engineering,Nanjing University,Nanjing 210023

出　　处：《CCS Chemistry》2023年第2期497-509,共13页中国化学会会刊（英文）

基　　金：supported by the National Key Research and Development Program of China(grant no.2018YFC0910301)from the Ministry of Science and Technology of China,the Key Grant(grant no.21834003)from the National Natural Science Foundation of China,and the Excellent Research Program of Nanjing University(grant no.ZYJH004)to Z.L.

摘　　要：High mannose oligosaccharides are characteristic and essential for immune evasion of many viruses and cancer cells.They are potential targets for viral inhibition and cancer diagnosis/therapy.Particularly,high mannose-binding reagents may be a unique asset for fighting the ongoing and mutating SARSCoV-2 virus.Lectins are prevailing reagents for saccharide binding but suffer from inadequate specificity and apparent immunogenicity.Meanwhile,other reagents for the same purpose,such as antibodies and aptamers,have rarely been reported.Herein,using molecularly imprinted magnetic nanoparticles as a potent platform,we report a smart selection method for fine screening of high mannose-specific aptamers.Monovalent aptamers were first effectively screened within eight rounds of selection.Multivalent aptamers,in the forms of dendritic polymer or tetrahedral DNA nanostructure(TDN),were further engineered.The aptamers exhibited high affinity toward the spike protein of SARS-CoV-2 and the envelope protein GP120 of HIV.Both the monovalent aptamer and its TDN form exhibited a certain inhibition effect to the SARS-CoV-2 pseudovirus.On the other hand,both the monovalent aptamer and its dendritic form permitted the recognition of cancer cells over normal cells.Therefore,as unprecedented reagents for broad-spectrum viral inhibition and cancer targeting,these aptamers hold great promise for clinical treatment and diagnosis.

关 键 词：APTAMER high mannose glycans VIRUS cancer molecular recognition 

分 类 号：R73[医药卫生—肿瘤]