热灭活嗜热链球菌MN002调节肠道菌群结构和胆汁酸改善高脂饲料诱导的肥胖小鼠脂代谢  

Heat-inactivated Streptococcus thermophilus MN002 alleviate lipid metabolism of high fat diet-fed induced obese mice through modulating gut microbiota structure and bile acids

作　　者：赵锦程 罗雅亭 李亚彭 程如越 沈曦[1] 陈建国 康小红[2] 李周勇[2] 何方[1] Zhao Jincheng;Luo Yating;Li Yapeng;Cheng Ruyue;Shen Xi;Chen Jianguo;Kang Xiaohong;Li Zhouyong;He Fang(West China School of Public Health and West China Fourth Hospital,Sichuan University,Chengdu 610041,China;Inner Mongolia Meng Niu Dairy(Group)Co.,Ltd,Huhhot 011500,China)

机构地区：[1]四川大学华西公共卫生学院/四川大学华西第四医院,成都610041 [2]内蒙古蒙牛乳业集团股份有限公司,呼和浩特011500

出　　处：《卫生研究》2023年第2期259-264,共6页Journal of Hygiene Research

摘　　要：目的观察热灭活嗜热链球菌MN-ZLW-002(MN002)对高脂饲料喂养肥胖小鼠糖脂代谢、肠道菌群以及胆汁酸的影响。方法60只3周龄雄性C57BL/6小鼠按体重随机分为对照组、高脂组、干预组3组,每组20只。适应性喂养1周后,对照组喂饲普通饲料,高脂组和干预组喂饲高脂饲料,干预组持续灌胃热灭活MN002(绝对含菌量109CFU/d)12周,对照组和高脂组灌胃等体积(0.2 mL/d)生理盐水。实验期间测量小鼠体重、摄食量、空腹血糖并收集粪便。实验结束时测量小鼠口服糖耐量并收集小鼠血液、肠周脂肪、睾周脂肪、肾周脂肪样本。苏木精-伊红染色观察肝脏组织病理学变化。全自动生化分析仪检测血清甘油三酯、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇以及总胆固醇含量,液相色谱-质谱法检测粪便中胆汁酸含量,16S rDNA测序技术分析小鼠肠道菌群结构。结果高脂饲料喂养小鼠12周后,高脂组和干预组小鼠体重、体脂肪均显著高于对照组(P<0.05,P<0.01);与高脂组相比,干预组小鼠的血清甘油三酯、总胆固醇、肾周脂肪显著降低(P<0.05);粪便中硫酸化石胆酸含量升高,熊去氧胆酸、猪去氧胆酸、脱氧胆酸含量降低(P<0.01)。热灭活MN002能够显著提高瘤胃梭菌属和另支菌属的相对丰度,降低乳杆菌属相对丰度(P<0.01)。结论热灭活嗜热链球菌MN002能够调节高脂饲料诱导的肥胖小鼠肠道菌群结构以及胆汁酸组成与含量,进而改善高脂饲料引起的脂代谢紊乱。OBJECTIVE To explore the effects of heat-inactivated Streptococcus thermophilus MN-ZLW-002(MN002)on glucose metabolism,lipid metabolism,gut microbiota and bile acids in high-fat diet fed obese mice.METHODS Sixty 3-week-old male C57BL/6 mice were randomly divided into control group,high-fat group and intervention group(n=20).After 1 week of adaptive feeding,the control group was fed with normal chow and continued intragastric administration of normal saline for 12 weeks,the high-fat group was fed with high-fat diet and continued intragastric administration of normal saline for 12 weeks,and the intervention group was fed with high-fat diet and continued intragastric of MN002 for 12 weeks.During the experiment,the body weight,food intake,fasting blood glucose content of mice were measured and feces were collected.At the end of the experiment,the oral glucose tolerance of mice was measured and blood,periintestinal fat,peritestosterone fat and perirenal fat samples were collected.The histopathological changes of liver were observed by hematoxylin-eosin staining.Triglyceride,low density lipoprotein,high density lipoprotein and total cholesterol were detected by automatic biochemical analyzer,bile acids content in feces was detected by liquid chromatography-mass spectrometry,gut microbiota structure of mice was analyzed by 16S rDNA sequencing.RESULTS Compared with high fat group,serum triglyceride,total cholesterol and perirenal fat in intervention group were significantly decreased(P<0.05),the content of fossil cholic acid sulfate in feces was significantly increased,while the content of ursodeoxycholic acid,porcine deoxycholic acid and deoxycholic acid were significantly decreased(P<0.01).Heat inactivation of MN002 could significantly increase the relative abundance of Ruminiclostridium and Alistipes and reduce the relative abundance of Lactobacillus(P<0.01).CONCLUSION Heat-inactivated Streptococcus thermophilus MN002 can regulate the gut microbiota structure and bile acid composition and content of high-fat diet fed

关 键 词：高脂饲料 胆汁酸 肠道菌群 嗜热链球菌 代谢紊乱 

分 类 号：R151.41[医药卫生—营养与食品卫生学]