难治性癫痫患儿血清lncRNA NKILA、miR-124表达及临床意义  

The expression and clinical significance of lncRNA NKILA and miR-124 in the serum of children with refractory epilepsy

作　　者：郑宇霞[1] 李亚玲[3] 沈璟[4] 周玲[3] 段丽芬[2] ZHENG Yuxia;LI Yaling;SHEN Jing;ZHOU Ling;DUAN Lifen(Neurology Department,Kunming Children's Hospital,Kunming 650000,Yunnan,China;Epilepsy Center,Kunming Children's Hospital,Kunming 650000,Yunnan,China;Sensory Control Department,Kunming Children's Hospital,Kunming 650000,Yunnan,China;Medical Records Department,Kunming Children's Hospital,Kunming 650000,Yunnan,China)

机构地区：[1]昆明市儿童医院神经内科,云南昆明650000 [2]昆明市儿童医院癫痫中心,云南昆明650000 [3]昆明市儿童医院感控科,云南昆明650000 [4]昆明市儿童医院病案科,云南昆明650000

出　　处：《中南医学科学杂志》2023年第2期214-217,共4页Medical Science Journal of Central South China

基　　金：云南省科技厅重大科技专项计划(202102AA100021);云南省科技厅昆明医科大学应用基础研究联合专项资金面上项目(202001AY070001-273);中国抗癫痫协会癫痫科研基金(CQ-B-2021-04);昆明市卫生科技人才培养项目暨“十百千”工程培养项目[2021-SW(省)-23]。

摘　　要：目的 探究难治性癫痫(RE)患儿血清lncRNA NKILA、miR-124的表达水平变化及临床意义。方法 选取收治的61例RE患儿作为RE组,63例可控性癫痫(CE)患儿为CE组,另选取同期本院体检健康儿童66例作为对照组。采用qRT-PCR法测定各组血清lncRNA NKILA、miR-124的表达水平;采用ROC分析血清lncRNA NKILA、miR-124对RE的诊断价值;采用多因素Logistic回归分析影响RE的危险因素。结果 与对照组比较,CE组患儿和RE组患儿血清lncRNA NKILA、miR-124水平明显升高(P<0.05)。与CE组比较,RE组患儿血清lncRNA NKILA、miR-124水平明显升高(P<0.05)。ROC曲线分析显示,lncRNA NKILA、miR-124诊断RE患儿的ROC曲线下面积分别为0.781、0.783。多因素Logistic回归分析表明,lncRNA NKILA、miR-124是RE发生的独立危险因素(P<0.05)。结论 lncRNA NKILA、miR-124的表达升高与RE的发生有关,可作为RE患儿诊断的血清学标志物。Aim To explore the changes in the expression levels of serum lncRNA NKILA and miR-124 in children with refractory epilepsy(RE)and their clinical significance.Methods 61 children with RE were selected as RE group and 63 children with controllable epilepsy(CE)as CE group,and 66 healthy children who came to the health department for physical examination during the same period were selected as control group.The expression levels of serum lncRNA NKILA and miR-124 in each group were determined by qRT-PCR.ROC was used to analyze the diagnostic value of serum lncRNA NKILA and miR-124 for RE;Multivariate Logistic regression was used to analyze the risk factors affecting RE.Results Compared with the control group,the serum lncRNA NKILA and miR-124 levels of children in the CE group and the RE group were significantly higher(P<0.05).Compared with the CE group,the levels of serum lncRNA NKILA and miR-124 in the RE group were significantly higher(P<0.05).ROC curve analysis showed that the area under the ROC curve of lncRNA NKILA and miR-124 for the diagnosis of RE children was 0.781 and 0.783;multivariate Logistic regression analysis showed that lncRNA NKILA and miR-124 were independent risk factors for the occurrence of RE(P<0.05).Conclusion The increased expression of lncRNA NKILA and miR-124 are related to the occurrence of RE,and they can be used as serum markers for the diagnosis of children with RE.

关 键 词：难治性癫痫 lncRNA NKILA miR-124 

分 类 号：R742.1[医药卫生—神经病学与精神病学]