食品能力验证样品中沙门氏菌的分离与多相分类鉴定  

Isolation and Polyphasic Taxonomy of Salmonella in Food Proficiency Testing Samples

作　　者：何肖云 饶秋华 吕新 刘兰英 傅建炜 HE Xiao-yun;RAO Qiu-hua;LV Xin;LIU Lan-ying;FU Jian-wei(Institute of Agricultural Quality Standards and Testing Technology,Fujian Academy of Agricultural Sciences/Fujian Key Laboratory of Agro-products Quality&Safety,Fuzhou,Fujian 350003,China)

机构地区：[1]福建省农业科学院农业质量标准与检测技术研究所/福建省农产品质量安全重点实验室,福建福州350003

出　　处：《福建农业科技》2023年第1期51-57,共7页Fujian Agricultural Science and Technology

基　　金：福建省科技计划——省属公益类科研院所基本科研专项(2019R1022-6);福建省农业科学院科技创新团队建设项目(CXTD2021011-3);福建省人民政府、中国农业科学院农业高质量发展超越“5511”协同创新工程(XTCXGC2021020)。

摘　　要：为沙门氏菌的快速检测与鉴定提供参考,从传统标准检测方法、特异性PCR、分子系统发育3个层面,其中传统标准检测方法基于食品安全国家标准GB 4789.4-2016《食品微生物学检验沙门氏菌检验》、特异性PCR基于invA基因、分子系统发育基于16S rDNA,对能力验证(CFAPA-805)样品进行沙门氏菌的分离鉴定。结果表明:3种检测方法结果一致,样品341中检出沙门氏菌,其中生化鉴定、血清学鉴定与分子系统发育鉴定结果显示,此次阳性样品中的沙门氏菌为沙门氏菌生化群(Ⅰ),肠炎沙门氏菌肠炎亚种,血清分型为O:9,12:H:g,m。本次实验室的能力验证结果为“满意”,肯定实验室沙门氏菌检测能力。通过比较,特异性PCR鉴定可以确定沙门氏菌是否检出,生化鉴定、血清学鉴定和分子系统发育鉴定可以确认沙门氏菌的生化群、血清分型与分子分型。In order to provide reference for the rapid detection and identification of Salmonella,Salmonella was isolated and identified from the proficiency testing(CFAPA-805)samples from three aspects including the traditional standard detection methods,specific PCR and molecular phylogeny.The traditional standard detection method was based on the national food safety standard(GB 4789.4-2016)″Salmonella Examination of Food Microbiological Examination″,the specific PCR was based on the invA gene,and the molecular phylogeny was based on the 16S rDNA.The results showed that the results of the three detection methods were consistent.Salmonella was detected in 341 samples,and the results of physiological and biochemical identification,serological identification and molecular phylogenetic identification showed that:the Salmonella in the positive samples was Salmonella biochemical group(Ⅰ),Salmonella enteritidis subspecies,of which the serotyping was O:9,12:H:g,m.The ability verification result of this laboratory was″satisfactory″,affirming the laboratory′s ability to detect Salmonella.By the comparison,the specific PCR identification could determine whether Salmonella was detected.The physiological and biochemical identification,serological identification and molecular phylogenetic identification could confirm the biochemical group,serotyping and molecular typing of Salmonella.

关 键 词：沙门氏菌 多相鉴定 血清鉴定 特异性PCR 分子系统发育 

分 类 号：TS207.4[轻工技术与工程—食品科学]