Mechanism of intervention of medicine combined with mild moxibustion on hypothyroidism rat model  

作　　者：WANG Hong-yang SUN Jia-zi LIU Qing-qing YAN Jing ZHANG Tian-sheng HAO Chong-yao 

机构地区：[1]Shanxi University of Traditional Chinese Medicine,Jinzhong 030619,China [2]Shanxi Moxibustion Hospital,Taiyuan 030006,China

出　　处：《Journal of Hainan Medical University》2023年第4期46-51,共6页海南医学院学报（英文版）

基　　金：Shanxi Provincial Natural Science Foundation Project(202203021211083);College Science and Technology Innovation Project of Shanxi Provincial Education Department(2020L044);Graduate Innovation Program of Shanxi University of Traditional Chinese Medicine(2021CX035)。

摘　　要：Objective:To explore the intervention effect of medicine combined with mild moxibustion on immune factor and Na/I symporter(NIS)in hypothyroidism rat model.Methods:The model was successfully made by intragastric administration of propylthiouracil(PTU)solution medicine and medicine combined with mild moxibustion groups were given levothyroxine sodium suspension 60μg/kg body weight by gavage,once a day.In the medicine combined with mild moxibustion group,mild moxibustion was applied in“Dazhui”,“Mingmen”,“Pishu”,“Shenshu”,and 10 minutes per point,once a day,and one day off every six days;four weeks in a row.The model group and medicine was fixed in the same way as the medicine combined with mild moxibustion group.The blank group received no treatment.The contents of thyrotropin-releasing hormone(TSH),tatalthyroxine(TT4),thyroid peroxidase antibody(TPOAb),thyroglobulin antibodies(TGAb),Interleukin-4(IL-4)and Interleukin-23(IL-23)in serum were determined by Enzyme Linked Immunosorbent Assay(ELISA).The content of NIS and the expression level of NISmRNA in thyroid tissues of each group were detected by immunohistochemistry and real-time polymerase chain reaction(R-T PCR).Results:Compared with the blank group,the contents of TSH,TPOAb,TGAb and IL-23 in the serum of rats in the model group were increased,the contents of IL-4 and TT4 were decreased,and the contents of NIS and NISmRNA in thyroid tissue were decreased,with statistical significance(P<0.01).Compared with model group,the contents of TSH,TPOAb,TGAb and IL-23 in serum of medicine group and medicine combined with mild moxibustion group were decreased,while the contents of IL-4 and TT4 were increased;the NIS content and NISmRNA expression in thyroid tissues were increased,and the differences were statistically significant(P<0.01).Compared with the medicine group,NISmRNA expression in thyroid tissues of medicine combined with mild moxibustion groups was increased,and the difference was statistically significant(P<0.05).Conclusion:Medicine combined w

关 键 词：HYPOTHYROIDISM INTERLEUKIN-4 INTERLEUKIN-23 Na/I symporter Medicine combined with mild moxibustion 

分 类 号：R285.5[医药卫生—中药学]