Lpcat3 deficiency promotes palmitic acid-induced 3T3-L1 mature adipocyte inflammation through enhanced ROS generation  

作　　者：Jiachun Hu Yan Deng Tingbo Ding Jibin Dong Yuning Liang Bin Lou 

机构地区：[1]Department of Pharmacology,School of Pharmacy,Fudan University,Shanghai 201203,China

出　　处：《Acta Biochimica et Biophysica Sinica》2023年第1期117-130,共14页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grant from the National Natural Science Foundation of China(No.31770864)。

摘　　要：Phosphatidylcholines(PCs)are major phospholipids in the mammalian cell membrane.Structural remodeling of PCs is associated with many biological processes.Lysophosphatidylcholine acyltransferase 3(Lpcat3),which catalyzes the incorporation of polyunsaturated fatty acyl chains into the sn-2 site of PCs,plays an important role in maintaining plasma membrane fluidity.Adipose tissue is one of the main distribution organs of Lpcat3,while the relationship between Lpcat3 and adipose tissue dysfunction during overexpansion remains unknown.In this study,we reveal that both polyunsaturated PC content and Lpcat3 expression are increased in abdominal adipose tissues of high-fat diet-fed mice when compared with chow-diet-fed mice,indicating that Lpcat3 is involved in adipose tissue overexpansion and dysfunction.Our experiments in 3T3-L1 adipocytes show that inhibition of Lpcat3 does not change triglyceride accumulation but increases palmitic acid-induced inflammation and lipolysis.Conversely,Lpcat3 overexpression exhibits anti-inflammatory and anti-lipolytic effects.Furthermore,mechanistic studies demonstrate that Lpcat3 deficiency promotes reactive oxygen species(ROS)generation by increasing NOX enzyme activity by facilitating the translocation of NOX4 to lipid rafts,thereby aggregating 3T3-L1 adipocyte inflammation induced by palmitic acid.Moreover,overexpression of Lpcat3 exhibits the opposite effects.These findings suggest that Lpcat3 protects adipocytes from inflammation during adipose tissue overexpansion by reducing ROS generation.In conclusion,our study demonstrates that Lpcat3 deficiency promotes palmitic acid-induced inflammation in 3T3-L1 adipocytes by enhancing ROS generation.

关 键 词：ADIPOCYTE INFLAMMATION lysophosphatidylcholine acyltransferase 3(Lpcat3) obesity 

分 类 号：R593.2[医药卫生—内科学]