Specific enrichment of urinary exosomes and exosomal glycopeptides by coefficient affinity of integrated L-cysteine and titania  

作　　者：Yijie Chen Haolin Chen Chenjie Yang Yonglei Wu Chunhui Deng Nianrong Sun 

机构地区：[1]Department of Chemistry,Institute of Metabolism&Integrate Biology(IMIB),Fudan University,Shanghai 200433,China [2]Department of Gastroenterology and Hepatology,Zhongshan Hospital,Fudan University,Shanghai 200032,China

出　　处：《Chinese Chemical Letters》2023年第2期254-258,共5页中国化学快报（英文版）

基　　金：supported by National Key R&D Program of China (No. 2018YFA0507501);the National Science Foundation for Distinguished Young Scholars of China (No. 21425518);the National Natural Science Foundation of China (Nos. 22074019, 22004017);Shanghai Sailing Program (No. 20YF1405300)。

摘　　要：Exosome and inclusive cargoes have manifested significant function in different biological events. In particular, glycopeptides in exosome are closely associated with occurrence and development of various diseases. Developing advanced tools is highly desired to enrich glycopeptides from exosomes, and enrich exosomes from complex biological samples as well. In this work, integration of L-cysteine and titania onto the surface of magnetic nanoparticles is designed to realize the coefficient affinity towards exosomes and inclusive glycopeptides. Benefiting from the synergistic affinity, we separate exosomes from human urine concentrate directly, which was proved by the detection of three typical antigen markers of exosomes. Furthermore, hardly any exosomes remained on materials after ultrasonication, which confirmed the good capture performance of Fe_(3)O_(4)@TiO_(2)@L-Cys and high release effect of direct lysis.Moreover, 146 glycopeptides corresponding to 77 glycoproteins were successfully identified from captured exosomes. These satisfactory results will inspire more efforts to be devoted to this field and will be extremely helpful to in-depth information excavation of biological markers, especially disease-related ones, through exosomes and inclusive glycopeptides.

关 键 词：GLYCOPEPTIDES ENRICHMENT Nanomaterials EXOSOMES Urine 

分 类 号：R446.12[医药卫生—诊断学]