模拟微重力条件下NRF2调节节律基因振荡作用  

The research of NRF2 in regulating clock gene oscillations under simulated microgravity conditions

作　　者：黄超 陈海龙[2] 王春艳[2] 冀国华[2] 吕柯[2] 元艳宏[2] 宋波[1] 曲丽娜[2] HUANG Chao;CHEN Hailong;WANG Chunyan;JI Guohua;LYU Ke;YUAN Yanhong;SONG Bo;QU Lina(Department of Pathology and Forensics,Dalian Medical University,Dalian 116044,China;State Key Laboratory of Space Medicine Fundamentals and Application,China Astronaut Research and Training Center,Beijing 100094,China)

机构地区：[1]大连医科大学病理学与法医学教研室,大连116044 [2]中国航天员科研训练中心航天医学基础与应用国家重点实验室,北京100094

出　　处：《生物学杂志》2023年第2期14-19,共6页Journal of Biology

基　　金：国家自然科学基金项目(No.21635001);航天医学基础与应用国家重点实验室项目(No.SMFA19K08)。

摘　　要：为探讨模拟微重力效应是否通过NRF2影响CLOCK和BMAL1表达节律振荡,使用细胞回转器模拟微重力效应,结果表明:模拟微重力效应使抗氧化转录因子NRF2表达水平显著降低,细胞内ROS水平显著增加;CLOCK和BMAL1表达显著降低,振荡节律减弱,周期缩短,相位改变,当过表达NRF2后可逆转微重力效应引起的节律变化,增强CLOCK和BMAL1的表达节律;提示模拟微重力效应可能通过NRF2调节CLOCK和BMAL1的表达振荡。To investigate whether the simulated microgravity effect affects the expression of CLOCK and BMAL1 through NRF2,the clinostat was used to simulate the microgravity effect.The results showed that the simulated microgravity effect significantly reduced the expression level of NRF2 and significantly increased intracellular ROS level.The expression of CLOCK and BMAL1 decreased significantly,the oscillation rhythm was weakened,the period was shortened,and the phase was changed.Then overexpression of NRF2 could reverse the rhythm changes caused by microgravity effect,and the expression rhythm of CLOCK and BMAL1 was enhanced.The results suggested that the simulated microgravity effect might regulate the expression oscillation of CLOCK and BMAL1 through NRF2.

关 键 词：昼夜节律 微重力 NRF2 CLOCK BMAL1 

分 类 号：Q786[生物学—分子生物学] R854[医药卫生—航空、航天与航海医学] R852.6[医药卫生—临床医学]