体外模拟消化筛选抗消化降糖肽及降糖活性研究  

Hypoglycemic Peptides of Anti-digestive by Simulated Digestionin Vitro and Hypoglycemic Activity

作　　者：韩迪 苏情 田晓闽 张学军 张西平 孙琳琳 龚魁杰[2] 郭玉秋[2] 刘开昌 陈利容[2] HAN Di;SU Qing;TIAN Xiaomin;ZHANG Xuejun;ZHANG Xiping;SUN Linlin;GONG Kuijie;GUO Yuqiu;LIU Kaichang;CHEN Lirong(Yantai University,Yantai,Shandong 264000,China;Crop Research Institute,Shandong Academy of Agricultural Sciences,Ji'nan,Shandong 250100,China;Qingdao Agricultural University,Qingdao,Shandong 266000,China;Shandong Tianjiu Biotechnology Co.,Ltd,Heze,Shandong 274108,China;Shandong Academy of Agricultural Sciences,Ji'nan,Shandong 250100,China)

机构地区：[1]烟台大学,山东烟台264000 [2]山东省农业科学院作物研究所,山东济南250100 [3]青岛农业大学,山东青岛266000 [4]山东天久生物技术有限公司,山东菏泽274108 [5]山东省农业科学院,山东济南250100

出　　处：《农产品加工》2023年第5期61-64,共4页Farm Products Processing

基　　金：山东省重点研发计划项目(医用食品专项,2019YYSP013);山东省泰山产业领军人才项目(2019.11)。

摘　　要：通过对豌豆肽、螺旋藻肽、海洋鱼肽、小麦肽、大豆肽、牡蛎肽和乳清肽7种降糖肽的体外模拟消化试验,对抗消化降糖肽进行筛选和降糖活性研究。结果表明,消化后的牡蛎肽和乳清肽的α-葡萄糖苷酶抑制率最高,分别为72.09%和69.91%。牡蛎肽和乳清肽的羟基自由基清除率IC_(50)值分别为5.40 mg/mL和7.32 mg/mL,DPPH自由基清除率IC_(50)值分别为6.66 mg/mL和2.53 mg/mL,总还原能力较高,均具有良好的抗氧化活性。牡蛎肽和乳清肽能够延缓人体餐后血糖上升,使餐后血糖浓度峰值降低,分别为8.63±0.15 mmol/L和8.23±0.15 mmol/L。In order to screen hypoglycemic peptides of anti-digestive and hypoglycemic activity,seven kinds of peptides including pea peptide,spirulina peptide,Marine fish peptide,wheat peptide,soybean peptide,oyster peptide and whey peptide were simulated in vitro.The results showed that theα-glucosidase inhibition rate of oyster peptide and whey peptide after digestion in vitro were 72.09%and 69.91%,which are the highest.The hydroxyl radical scavenging IC_(50) values of oyster peptide and whey peptide were 5.40 mg/mL and 7.32 mg/mL,and DPPH radical scavenging IC_(50) values were 6.66 mg/mL and 2.53 mg/mL,and the total reduction capacity was higher,so they both had good antioxidant activity.Oyster peptide and whey peptide could delay the rise of postprandial blood glucose and reduce the peak of postprandial blood glucose,which were 8.63±0.15 mmol/L and 8.23±0.15 mmol/L.

关 键 词：体外模拟消化 降糖肽 抗氧化活性 降糖活性 

分 类 号：R114[医药卫生—卫生毒理学]