饮料中甜菊糖苷水解条件的优化及高效液相色谱法测定  

Optimization of Acid Hydrolysis Conditions of Stevioside in Beverage and Determination of Steviol by High Performance Liquid Chromatography

作　　者：刘莹 李莹 刘祥 李长燕 王国宇 羊银 张瑀 LIU Ying;LI Ying;LIU Xiang;LI Chang-yan;WANG Guo-yu;YANG Yin;ZHANG Yu(Tianjin Institute for Food Safety Inspection Technology,Tianjin 300310,China;Animal Law Enforcement Team of Agricultural Comprehensive Administrative Law Enforcement Brigade,Dehui 130300,Jilin,China)

机构地区：[1]天津市食品安全检测技术研究院,天津300310 [2]吉林省德惠市农业综合行政执法大队畜牧执法队,吉林德惠130300

出　　处：《食品研究与开发》2023年第8期177-182,共6页Food Research and Development

基　　金：天津市市场和质量监督管理委员会科技计划项目(2018-W05)。

摘　　要：该文建立高效液相色谱(high performance liquid chromatography,HPLC)法测定饮料中甜菊醇的定量分析方法,并优化饮料中甜菊糖苷水解成甜菊醇的条件。采用3 mmol/g路易斯酸水解,无水乙醇提取,以70%乙腈水溶液(体积分数)作为流动相,使用ZORBAX SB-C18色谱柱。结果表明,甜菊醇在5.0μg/mL~100.0μg/mL的浓度范围内线性关系较好(r2≥0.999),平均回收率在81.9%~92.3%,相对标准偏差在1.17%~3.67%。饮料中甜菊糖苷转化甜菊醇的最佳工艺条件:催化剂为氯化铁,提取温度100℃,甜菊糖苷浓度300 mg/mL,提取时间3 h,催化剂含量3 mmol/g。A high performance liquid chromatography(HPLC)method was developed for the determination of steviol in beverage,and the conditions for the hydrolysis of stevioside into steviol were optimized.Specifically,the samples were hydrolyzed by 3 mmol/g Lewis acid and then extracted by 100%ethanol.HPLC was performed with ZORBAX SB-C18 column and 70%(volume fraction)acetonitrile aqueous solution as the mobile phase.The result suggested that the steviol had good linear relationship(r2≥0.999)within the concentration range of 5.0μg/mL-100.0μg/mL.The average recovery ranged from 81.9%to 92.3%,and the RSD from 1.17%to 3.67%.The hydrolysis conditions were optimized as hydrolysis with 3 mmol/g ferric chloride as the catalyst and the stevioside concentration of 300 mg/mL at 100℃for 3 h.

关 键 词：路易斯酸 甜菊糖苷 甜菊醇 高效液相色谱法 酸水解 

分 类 号：TS27[轻工技术与工程—农产品加工及贮藏工程] O657.72[轻工技术与工程—食品科学与工程]