高温大曲中一株高产酸细菌的筛选与生物学特性分析  

Screening and Biological Characteristics Analysis of a High Acid Producing Bacterium in High-Temperature Daqu

作　　者：蒋泽元 周明茂 李欢 JIANG Zeyuan;ZHOU Mingmao;LI Huan(Department of Brewing Engineering,Moutai Institute,Renhuai,Guizhou 564507,China;Department of Tourism Management,Moutai Institute,Renhuai,Guizhou 564507,China)

机构地区：[1]茅台学院酿酒工程系,贵州仁怀564507 [2]茅台学院旅游管理系,贵州仁怀564507

出　　处：《西昌学院学报（自然科学版）》2023年第1期11-16,共6页Journal of Xichang University(Natural Science Edition)

基　　金：茅台学院高层次人才科研启动基金(mygccrc[2022]015)。

摘　　要：产酸菌是高温大曲中的一类重要细菌微生物,其主要作用是在酿酒过程中通过发酵为白酒提供香味成分和增加白酒口感风味,目前对高温大曲中产酸菌特性的研究较少。用高温大曲通过富集培养、钙平板分离纯化获得酱香大曲细菌菌株7株;通过定性定量试验,发现命名为RS-6的菌株产酸量最高,达到2.13 g/L;通过耐乙醇、耐乙酸、耐盐和耐高温试验进一步对RS-6菌株进行研究,发现该菌株的最高乙醇耐受质量分数为7%,最高乙酸耐受质量分数为3%,最高盐耐受质量分数为0.8%,最适生长温度为30℃。研究表明,从高温大曲中筛选到一株产酸量高、乙醇耐受性高和耐盐性高的产酸细菌。Acid producing bacteria are a kind of important bacteria and microorganisms in high-temperature Daqu.Their main role is to provide flavor components for liquor and increase liquor flavor through fermentation in the process of liquor brewing.However,there are few studies on the characteristics of acid producing bacteria in high-temperature Daqu.In this study,7 strains were obtained by preliminary screening through enrichment culture and calcium plate from Hightemperature Daqu.Through qualitative and quantitative experiments,it was found that RS-6 strain had the highest acid production,reaching 2.13 g/L.The strain RS-6 was further studied by ethanol resistance,acetic acid resistance,salt resistance and high temperature resistance experiments.It was found that the strain had the quality of 7%ethanol resistance,3%acetic acid resistance,0.8%salt resistance and its optimum growth temperature was 30℃.The study indicated that a strain of acid producing bacteria with high acid production,high ethanol tolerance and high salt tolerance was screened from high temperature Daqu.

关 键 词：大曲 产酸菌 分离纯化 生物特性 

分 类 号：TS261.13[轻工技术与工程—发酵工程]