Breakthrough in efficient cloning and activation of large cryptic biosynthetic gene clusters from high GC actinobacteria  

作　　者：Jun-Yi Wang Bing-Zhi Li 

机构地区：[1]Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education),School of Chemical Engineering and Technology,Tianjin University,Tianjin,300072,PR China

出　　处：《Synthetic and Systems Biotechnology》2022年第4期1064-1065,共2页合成和系统生物技术（英文）

摘　　要：Synthetic genomics,as one of the important parts of synthetic biology,has become a hotspot in recent years,and several bacterial genomes and yeast chromosomes have been designed and synthesized from de novo.However,during genome synthesis,one technical challenge is the efficient cloning and editing of large genome fragments.At the same time,with the advent of the genomics era,actinobacteria(e.g.Streptomyces)is again regarded as a rich source for novel natural products.However,most of Streptomyces and nearly half the actinomycetes are high GC content.

关 键 词：FRAGMENTS ACTIVATION CLONING 

分 类 号：Q78[生物学—分子生物学]