Simvastatin-hydroxyapatite coatings prevent biofilm formation and improve bone formation in implant-associated infections  

作　　者：Tiantong Sun Jie Huang Wang Zhang Xuanqi Zheng Hong Wang Jing Liu Huijie Leng Wanqiong Yuan Chunli Song 

机构地区：[1]Department of Orthopedics,Peking University Third Hospital,Beijing,China [2]Beijing Key Laboratory of Spinal Diseases,Beijing,China [3]Engineering Research Center of Bone and Joint Precision Medicine,Ministry of Education,Beijing,China [4]Institute of Medical Sciences,The Second Hospital of Shandong University,Jinan,China

出　　处：《Bioactive Materials》2023年第3期44-56,共13页生物活性材料（英文）

基　　金：National Key Research and Development Program[Grant No.2020YFC2009004];National Natural Science Foundation of China(Project No.81874010);PKU-Baidu Fund(Grant No.2020BD014).

摘　　要：Implant-associated infections(IAIs)caused by biofilm formation are the most devastating complications of orthopedic surgery.Statins have been commonly and safely used drugs for hypercholesterolemia for many years.Here,we report that simvastatin-hydroxyapatite-coated titanium alloy prevents biofilm-associated infections.The antibacterial properties of simvastatin against Staphylococcus aureus and Staphylococcus epidermidis biofilms in vitro was confirmed by crystal violet staining and live-dead bacterial staining.We developed a simvastatin-and hydroxyapatite(Sim-HA)-coated titanium alloy via electrochemical deposition.Sim-HA coatings inhibited Staphylococcus aureus biofilm formation and improved the biocompatibility of the titanium alloy.Sim-HA coatings effectively prevented Staphylococcus aureus IAI in rat femurs,as confirmed by radiological assessment and histological examination.The antibacterial effects of the Sim-HA coatings were attributed to their inhibitory effects on biofilm formation,as verified by scanning electron microscopic observations and bacterial spread plate analysis.In addition,the Sim-HA coatings enhanced osteogenesis and osteointegration,as verified by micro-CT,histological evaluation,and biomechanical pull-out tests.In summary,Sim-HA coatings are promising implant materials for protection against biofilm-associated infections.

关 键 词：Implant-associated infections SIMVASTATIN Antibacterial BIOFILM OSTEOGENESIS 

分 类 号：R318.08[医药卫生—生物医学工程]