玉米低聚肽改善肝纤维化作用机制研究及其在饮料中的应用  

Study on the Mechanism of Corn Oligopeptides Improving Liver Fibrosis and Its Application in Liver Protection Drink

作　　者：冯晓文 方磊 卢志浩 程青丽 谷瑞增 潘兴昌 李国明 刘文颖 FENG Xiao-wen;FANG Lei;LU Zhi-hao;CHENG Qing-li;GU Rui-zeng;PAN Xing-chang;LI Guo-ming;LIU Wen-ying(China National Research Institute of Food and Fermentation Industries Co.Ltd.,Beijing Engineering Research Center of Protein and Functional Peptides,Beijing 100015,China)

机构地区：[1]中国食品发酵工业研究院有限公司,北京市蛋白功能肽技术研究中心,北京100015

出　　处：《饮料工业》2023年第2期1-7,共7页Beverage Industry

基　　金：“十三五”国家重点研发计划(2016YFD0400604);国家自然科学基金(31671963);北京市科技创新基地培育与发展工程专项(Z191100002819001)。

摘　　要：为研究玉米低聚肽对肝纤维化的影响,以昆明小鼠为研究对象,灌胃CCl4建立肝纤维化模型,灌胃不同剂量的玉米低聚肽溶液给予治疗,同时给予1mg/kg剂量的秋水仙碱作为阳性对照。通过苏木素-伊红(hematoxylineosin,HE)染色和Masson三色染色评估肝纤维化小鼠的肝损伤和胶原沉积;通过酶联免疫吸附测定法(enzyme linked immunosorbent assay,ELISA)、反转录聚合酶链反应(reverse transcription PCR,RT-PCR)和蛋白免疫印迹法(western blotting,WB)研究玉米低聚肽对肝纤维化的改善机制。HE染色和Masson三色染色结果表明玉米低聚肽能够使受损的肝组织结构恢复正常,且减少了肝组织胶原沉积。此外,ELISA、RT-PCR和WB结果表明玉米低聚肽通过降低促炎因子水平和减少细胞外基质的产生从而改善肝纤维化。上述结果表明玉米低聚肽具有护肝的效果,且基于玉米低聚肽的护肝效果进一步研究一种以玉米低聚肽为核心原料的有益于肝脏的饮料配方,为其作为新型益肝饮料的开发提供了理论支撑。To study the effect of corn oligopeptides(COPs)on liver fibrosis,Kunming(KM)mice were given CCl4 intragastric administration to establish liver fibrosis model.COPs solution at different doses was given intragastric administration,and colchicine at 1mg/kg was given as positive control.Hematoxylin-eosin(HE)staining and Masson trichrome staining were used to evaluate liver injury and collagen deposition in liver fibrosis mice.The improvement mechanism of COPs on liver fibrosis was investigated by enzyme linked immunosorbent assay(ELISA),reverse transcription PCR(RT-PCR)and western blotting(WB).COPs could restore the damaged liver tissue structure to normal and reduce collagen deposition in liver tissue as suggested by HE staining and Masson trichrome staining.In addition,the results of ELISA,RT-PCR and WB showed that COPs improved liver fibrosis by reducing proinflammatory cytokine levels and extracellular matrix production.The above results indicated that COPs have the effect of protecting liver.And based on the liver protecting effect of COPs,a liver protection drink formula with COPs as the core raw material was further studied,which provides theoretical support for its development as a new type of liver protecting drink.

关 键 词：玉米低聚肽 肝纤维化 促炎因子 胶原 护肝饮料 

分 类 号：TS201.4[轻工技术与工程—食品科学]