环二鸟苷酸调控细菌胞外多糖生物合成的研究进展  

Research Progress on Regulation of Bacterial Exopolysaccharide Biosynthesis by Cyclic Diguanylate

作　　者：于连升 葛菁萍[1,2] 平文祥 杜仁鹏[1,2] YU Liansheng;GE Jingping;PING Wenxiang;DU Renpeng(School of Life Sciences,Heilongjiang University,Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education,Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region,Key Laboratory of Microbiology,College of Heilongjiang Province,Harbin 150080,China;Hebei University of Environmental Engineering,Hebei Key Laboratory of Agroecological Safety,Qinhuangdao 066102,China)

机构地区：[1]黑龙江大学生命科学学院,农业微生物技术教育部工程研究中心,黑龙江省寒区植物基因与生物发酵重点实验室,黑龙江省普通高校微生物重点实验室,黑龙江哈尔滨150080 [2]河北环境工程学院,河北省农业生态安全重点实验室,河北秦皇岛066102

出　　处：《食品工业科技》2023年第9期422-430,共9页Science and Technology of Food Industry

基　　金：黑龙江省自然科学基金优秀青年基金项目(YQ2021C030);中国博士后科学基金资助项目(2022MD713755);黑龙江省博士后资助项目(LBH-Z21082);‘新时代龙江优秀硕士、博士学位论文’项目(LJYXL2022-020);黑龙江省省属高等学校基本科研业务费科研项目(2022-KYYWF-1075)。

摘　　要：细菌胞外多糖(Exopolysaccharide,EPS)是细菌生长代谢过程中自身合成并分泌到细胞壁外的一种次级代谢产物,可以调节细胞对不同基质的初始附着,保护细胞抗环境胁迫和脱水。作为潜在的益生元,EPS具有安全,无毒和特殊的理化性质,广泛应用在食品、医药、生物和工业等领域。然而,细菌代谢系统复杂,EPS的生物合成机制仍未得到全面解析。环二鸟苷酸(Cyclic diguanylate,c-di-GMP)作为一类重要的第二信使,在细菌的生物被膜形成、运动性、黏附、毒力以及EPS合成等众多生理活动上发挥重要的调控作用。c-di-GMP转录调控机制的解析,为探明细菌EPS的生物合成机理提供了全新的思路。本文详细总结了c-di-GMP的特性及合成降解途径,重点综述c-di-GMP在介导细菌EPS生物合成过程中的调控机理。本篇综述为揭示细菌EPS生物合成机理和构效关系的研究提供理论基础。Bacterial exopolysaccharide(EPS)is a secondary metabolite that is synthesized and secreted outside the cell wall during bacterial growth and metabolism.It can regulate the initial attachment of cells to different substrates and protect cells against environmental stress and dehydration.As a potential prebiotic,EPS has the characteristics of safety,non-toxicity and unique physical and chemical properties,and is widely used in the fields of food,medicine,biology and industry.However,the bacterial metabolic system is complex,and the biosynthetic mechanism of EPS has not been fully elucidated.Cyclic diguanylate(c-di-GMP)is an important second messenger,and it plays an important role in the regulation of many physiological activities such as biofilm formation,motility,adhesion,virulence and EPS synthesis.The analysis of the transcriptional regulation mechanism of c-di-GMP provides a new idea for exploring the biosynthesis mechanism of bacterial EPS.This paper summarizes the characteristics and synthetic degradation pathways of c-di-GMP in detail,and focuses on the regulatory mechanism of c-di-GMP in the process of bacterial EPS biosynthesis.This paper provides a theoretical basis for revealing the mechanism of bacterial EPS biosynthesis and the structure-activity relationship.

关 键 词：细菌 胞外多糖 生物合成 环二鸟苷酸 

分 类 号：Q939.97[生物学—微生物学]