In-Depth Metaproteomics Analysis of Oral Microbiome for Lung Cancer  

作　　者：Xiaoteng Jiang Yan Zhang Huiyu Wang Zeyuan Wang Shen Hu Chengxi Cao Hua Xiao 

机构地区：[1]state Key Laboratory of Microbial Metabolism,Joint International Research Laboratory of Metabolic Developmental Sciences,School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China [2]School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China [3]School of Dentistry and Jonsson Comprehensive Cancer Center,University of California-Los Angeles,Los Angeles 90095,USA [4]Department of Instrument Science and Engineering,School of Electronic Information and Electrical Engineering,Shanghai Jiao Tong University,Shanghai 200240,China

出　　处：《Research》2023年第1期313-330,共18页研究（英文）

基　　金：supported by grants from the Natural Science Foundationn of Shanghai(nos.21ZR1433200and 19ZR1427800);the National Key Research and Development Program of China(no.2017YFC1200204);the National Natural Science Foundation of China(nos.21675110 and 31727801);the Key Scientific Project of Shanghai Jiao Tong University(nos.TMSK-2020-130 and YG2017MS80).

摘　　要：The human oral microbiome correlates with numerous diseases,including lung cancer.Identifying the functional changes by metaproteomics helps understand the disease-related dysbiosis,yet characterizing low-abundant bacteria is challenging.Here,we developed a free-flow isoelectric focusing electrophoresis-mass spectrometry-(FFIEF-MS-)based metaproteomics strategy to reduce host interferences and enrich low-abundant bacteria for in-depth interpretation of the oral microbiome.With our method,the number of interfering peptides decreased by 52.87%,whereas the bacterial peptides and species increased by 94.97%and 44.90%,respectively,compared to the conventional metaproteomics approach.We identified 3647 bacterial proteins,which is the most comprehensive oral metaproteomics study to date.Lung cancer-associated bacteria were validated among an independent cohort.The imbalanced Fusobacterium nucleatum and Prevotella histicola and their dysregulated functions in inhibiting immune response and maintaining cell redox homeostasis were revealed.The FFIEF-MS may serve as a valuable strategy to study the mechanisms between human diseases and microbiomes with broader applications.

关 键 词：LUNG diseases HOMEOSTASIS 

分 类 号：R73[医药卫生—肿瘤]