基于生物信息学分析SUFU基因在胃癌组织中的表达及意义  

Bioinformatics-Based Analysis of the Expression and Significance of SUFU Gene in Gastric Cancer Tissues

作　　者：杨波[1] 何磊[1] 付田丽[1] 程玉[2] 王雪梅[2] 刘娟[1] YANG Bo;HE Lei;FU Tianli(Chengde Central Hospital,Hebei Chengde 067000,China)

机构地区：[1]河北省承德市中心医院,河北承德067000 [2]承德医学院病理教研室,河北承德067000

出　　处：《河北医学》2023年第4期551-555,共5页Hebei Medicine

基　　金：承德医学院高层次人才科研启动基金,(编号:202201)。

摘　　要：目的:多种数据库结合Real-time PCR实验分析SUFU基因在胃癌组织中的表达情况,并探讨其表达差异的临床意义。方法:利用Oncomine数据库分析SUFU基因在胃癌中的表达情况,TGCA数据库在线工具UALCAN分析SUFU基因在正常胃组织和胃癌组织中表达差异及与胃癌临床病理特征的关系,Kaplan-Meier Plotter数据库分析SUFU基因表达与胃癌患者预后的相关性,并收集临床样本,通过PCR实验进行验证。结果:对Oncomine数据库中12项关于胃癌的研究进行Meta分析,SUFU差异表达基因中位数排名为1560.0,P=0.002,其中5项研究显示SUFU在胃癌组织中的表达明显高于癌旁正常组织。UALCAN同样显示SUFU在胃癌组织中高表达,并且SUFU高表达与患者的年龄及肿瘤分化程度相关:低龄组SUFU表达水平高于高龄组;低分化组中SUFU的表达水平高于高分化组及中分化组(P<0.05)。Kaplan-Meier Plotter数据库显示SUFU表达水平与胃癌患者预后显著相关(P<0.05),SUFU高表达患者总生存率低于低表达患者。PCR实验也验证SUFU基因在胃癌组织中高表达。结论:SUFU在胃癌组织高表达,且与胃癌患者不良预后相关。Objective:Multiple databases combined with real-time PCR assay were used to analyze the expression of SUFU gene in gastric cancer tissues,and to explore its clinical significance.Methods:The Oncomine database was used to analyze the expression of SUFU gene in gastric cancer,and UALCAN,an online tool of TGCA database,was used to analyze the difference of SUFU gene expression between normal gastric tissues and gastric cancer tissues and the relationship between SUFU gene expression and clinicopathological features of gastric cancer.Kaplan-Meier Plotter database was used to analyze the correlation between SUFU gene expression and prognosis of gastric cancer patients,and clinical samples were collected and verified by PCR experiment.Results:Meta-analysis of 12 studies on gastric cancer in Oncomine database showed that the median ranking of SUFU differentially expressed genes was 1560.0(P=0.002),among which 5 studies showed that the expression of SUFU in gastric cancer tissues was significantly higher than that in adjacent normal tissues.UALCAN also showed that SUFU was highly expressed in gastric cancer tissues,and the high expression of SUFU was correlated with the age of patients and the degree of tumor differentiation.The expression level of SUFU in the younger group was higher than that in the older group.The expression level of SUFU in low differentiation group was higher than that in high differentiation group and medium differentiation group(P<0.05).Kaplan-Meier Plotter database showed that the expression level of SUFU was significantly correlated with the prognosis of gastric cancer patients(P<0.05),the overall survival rate of patients with high SUFU expression was lower than that of patients with low SUFU expression.PCR also verified that SUFU gene was highly expressed in gastric cancer tissues.Conclusion:SUFU is highly expressed in gastric cancer tissues and is associated with poor prognosis in gastric cancer patients.

关 键 词：胃癌 生物信息学 SUFU 信号通路 

分 类 号：Q811.4[生物学—生物工程] R735.2[医药卫生—肿瘤]