Remodeling of the osteoimmune microenvironment after biomaterials implantation in murine tibia: Single-cell transcriptome analysis  

作　　者：Jia Li Congrui Zhao Yangbo Xu Lu Song Yanqi Chen Yuzi Xu Yang Ma Siyuan Wang Antian Xu Fuming He 

机构地区：[1]Department of Prosthodontics,Stomatology Hospital,School of Stomatology,Zhejiang University School of Medicine,Zhejiang Provincial Clinical Research Center for Oral Diseases,Key Laboratory of Oral Biomedical Research of Zhejiang Province,Cancer Center of Zhejiang University,Hangzhou,China

出　　处：《Bioactive Materials》2023年第4期404-422,共19页生物活性材料（英文）

基　　金：The study was supported by grants from the National Natural Science Foundation of China(No.82271026);the Key Research and Development Program of Science and Technology Department of Zhejiang Province(No.2019C03081).

摘　　要：Osseointegration seems to be a foreign body reaction equilibrium due to the complicated interactions between the immune and skeletal systems.The heterogeneity of the osteoimmune microenvironment in the osseointegration of implant materials remains elusive.Here,a single-cell study involving 40043 cells is conducted,and a total of 10 distinct cell clusters are identified from five different groups.A preliminary description of the osteoimmune microenvironment revealed the diverse cellular heterogeneity and dynamic changes modulated by implant properties.The increased immature neutrophils,Ly6C+CCR2hi monocytes,and S100a8hi macrophages induce an aggressive inflammatory response and eventually lead to the formation of fibrous capsule around the stainless steel implant.The enrichment of mature neutrophils,FcgR1hi and differentiated immunomodulatory macrophages around the titanium implant indicates favorable osseointegration under moderate immune response.Neutrophil-depletion mice are conducted to explore the role of neutrophils in osseointegration.Neutrophils may improve bone formation by enhancing the recruitment of BMSCs via the CXCL12/CXCR3 signal axis.These findings contribute to a better knowledge of osteoimmunology and are valuable for the design and modification of‘osteoimmune-smart’biomaterials in the bone regeneration field.

关 键 词：OSTEOIMMUNOLOGY NEUTROPHIL Implant OSSEOINTEGRATION Single-cell transcriptomics 

分 类 号：R318.08[医药卫生—生物医学工程]