葡萄蒸馏酒对高尿酸血症模型小鼠肠道菌群的调节作用  

Regulatory Effect of Distilled Spirit Made from Grapes on the Gut Flora of Hyperuricemic Mice

作　　者：付喜华 韩四海[1,2,3] 刘建学 李佩艳[1,2,3] 郭金英 罗登林[1,2,3] 岳崇慧 FU Xihua;HAN Sihai;LIU Jianxue;LI Peiyan;GUO Jinying;LUO Denglin;YUE Chonghui(College of Food and Bioengineering,Henan University of Science and Technology,Luoyang 471023,China;Henan Engineering Research Center of Food Material,Luoyang 471023,China;National Experimental Teaching Demonstration Center for Food Processing and Safety,Luoyang 471023,China)

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471023 [2]河南省食品原料工程技术研究中心,河南洛阳471023 [3]食品加工与安全国家级实验教学示范中心,河南洛阳471023

出　　处：《食品科学》2023年第7期161-168,共8页Food Science

基　　金：国家自然科学基金面上项目(31471658)。

摘　　要：为了探讨葡萄蒸馏酒与高尿酸血症模型小鼠肠道菌群的关系,将6周龄SPF级雄性小鼠随机分为52%葡萄蒸馏酒低剂量组、中剂量组、高剂量组、空白对照组、模型组和乙醇组,连续灌胃7 d后测定小鼠粪便中的短链脂肪酸和肠道微生物。利用Illumina MiSeq平台进行高通量测序,检测小鼠粪便16S rDNA基因,分析小鼠肠道菌群的变化,并使用气相色谱-质谱联用仪检测小鼠粪便中短链脂肪酸的含量变化。研究发现,低、中剂量葡萄蒸馏酒能提高高尿酸血症模型小鼠肠道微生物的多样性,显著增加拟杆菌门(Bacteroidota)的相对丰度(P<0.05),显著降低厚壁菌门(Firmicutes)的相对丰度(P<0.05),改善高尿酸血症模型小鼠的菌群失调及厚壁菌门与拟杆菌门比值的升高,并增加了肠道有益菌如丁蓖麻球菌、瘤胃球菌、罗氏菌、厌氧棍状菌、梭菌等的相对丰度;葡萄蒸馏酒中剂量组的乙酸、丙酸和正丁酸含量显著高于模型组和乙醇组(P<0.05)。结论:每日摄入52%葡萄蒸馏酒1 mL/kg m_(b)或2 mL/kg m_(b)可以改善高尿酸血症模型小鼠肠道菌群失调,对其肠道微生态具有积极的调节作用。This study was conducted in order to explore the effect of distilled spirit(with 52%alcohol content,V/V)made from grapes on the intestinal flora of hyperuricemic mice.Six-week-old specific pathogen free(SPF)male mice were randomly divided into low-dose,medium-dose,high-dose distilled spirit,control,hyperuricemic model and ethanol groups.After continuous gavage administration for seven days,the content of short chain fatty acids in mouse feces was determined by gas chromatography mass spectrometry(GC-MS).and gut microbiota composition was analyzed by 16S rDNA gene high-throughput sequencing on the Illumina MiSeq platform.Results indicated that the distilled spirit at low and medium doses could improve the diversity of gut microorganisms,significantly increase the relative abundance of Bacteroidota(P<0.05),reduce the relative abundance of Firmicutes(P<0.05),improve intestinal dysbacteriosis,increase Firmicutes to Bacteroidota ratio,and enhance the relative abundance of intestinal beneficial bacteria such as Butyricicoccus,Ruminococcaceae NK4A214_group,Roseburia,Anaerotruncus,and Clostridiaceae in hyperuricemic mice.Meanwhile,the contents of acetic acid,propionic acid and n-butyric acid in the medium-dose distilled spirit group were significantly higher than those in the model and ethanol groups(P<0.05).Therefore,daily intake of the distilled spirit at 1 or 2 mL/kg mb can improve the imbalance of gut flora in hyperuricemic mice and have a positive regulatory effect on the intestinal microecology.

关 键 词：葡萄蒸馏酒 高尿酸血症模型 肠道菌群 短链脂肪酸 气相色谱-质谱法 

分 类 号：TS201.4[轻工技术与工程—食品科学]