Carboxymethylation of the polysaccharide from the fermentation broth of Marasmius androsaceus and its antidepressant mechanisms  

作　　者：Yu Qiu Yan Su Jia Song Fangming Mou Jia Gou Xiaoqi Geng Xinyue Li Zhiqiang Nie Jianxin Wang Yu Zheng Min Wang 

机构地区：[1]State Key Laboratory of Food Nutrition and Safety,Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control,College of Biotechnology,Tianjin University of Science&Technology,Tianjin 300457,China [2]Key Laboratory of Chemical Biology and Molecular Engineering,Ministry of Education,Institute of Biotechnology,Shanxi University,Taiyuan 030006,China [3]Wisconsin Center for NanoBioSystems,School of Pharmacy,University of Wisconsin-Madison,Madison,WI 53706,USA [4]Pharmaceutical Sciences Division,School of Pharmacy,University of Wisconsin-Madison,Madison,WI 53706,USA

出　　处：《Food Science and Human Wellness》2023年第6期2417-2427,共11页食品科学与人类健康（英文）

基　　金：funded by the Natural Science Foundation of China(32072203);the Tianjin Municipal Education Commission(TD13-5013);the Open Project of State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science&Technology(SKLFNS-KF-202102);Natural Science Foundation of Shanxi(201901D211130);Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(2017105);Tianjin synthetic biotechnology innovation capacity improvement project(TSBICIP-KJGG-016);Tianjin Science and Technology Plan Project(21ZYJDJC00030).

摘　　要：To investigate the structure-activity relationship of polysaccharide and obtain a better antidepressant polysaccharide,the antidepressant-like activity of a carboxymethyl polysaccharide(C-MEPS2)subjected to submerged fermentation was systematically studied.PC12-H cell and Kunming mice were used to investigate the differences and their mechanism in the antidepressant effects of C-MEPS2 and MEPS2.Cell experiments have showed that C-MEPS2 has a better antidepressant effect than MEPS2.C-MEPS2 could exert antidepressant effects related to catecholamine synthesis with specifi c sites of TH,D2DR,and P-CAMKII.In addition,C-MEPS2 could repair the Res-induced damage in PC12-H cell,stabilize the mitochondrial membrane potential and regulate intracellular Ca^(2+) concentration,thus reducing cell apoptosis caused by RES.Antagonists common dosing experiments on animals further proved that CMEPS2 could signifi cantly improve the antidepressant effect of derivatives without affecting the antidepressant mechanism of MEPS2.It is speculated that it may be related to carboxymethylated modifi cation.

关 键 词：Carboxymethyl polysaccharide ANTI-DEPRESSANT CATECHOLAMINE 

分 类 号：TS201.4[轻工技术与工程—食品科学]