健康大学生尿道外口酵母菌带菌率调查及药敏分析  

Investigation on the Carrier Rate and Antifungal Susceptibility of Yeast Species Isolated from External Urethral Meatus of Healthy College Students

作　　者：白宗凯 吕能 刘润花 罗元希 吴秋玥 徐静 武有聪 Bai Zongkai;LüNeng;Liu Runhua;Luo Yuanxi;Wu Qiuyue;Xu Jing;Wu Youcong(Pre-clinical College,Dali University,Dali,Yunnan671000,China)

机构地区：[1]大理大学基础医学院,云南大理671000

出　　处：《大理大学学报》2023年第4期34-38,共5页Journal of Dali University

基　　金：国家自然科学基金项目(82060380,81660346);云南省中青年学术和技术带头人后备人才项目(202205AC160092)。

摘　　要：目的:了解健康大学生尿道外口酵母菌带菌率、菌种分布及其对常用抗真菌药物的敏感性。方法:随机抽取云南省某高校健康大学生261名,采集尿道外口拭子进行酵母菌分离培养、鉴定和药物敏感性实验并进行统计分析。结果:分离出酵母菌39株,总携带率为14.9%;261份标本中男性130份、女性131份,男性标本分离出酵母菌6株,携带率为4.6%,女性标本分离出酵母菌33株,携带率为25.2%,不同性别人群携带率差异有统计学意义(χ2=21.73,P=0.005);39株酵母菌中白假丝酵母菌11株(占28.2%)、光滑假丝酵母菌24株(占61.5%)、其他假丝酵母菌4株(占10.3%)。分离出的酵母菌对氟康唑、氟胞嘧啶和两性霉素B的药物敏感性几何均数分别为22.03、0.16、4.37μg/mL,耐药率分别为15.4%、0.0%和100.0%。结论:健康大学生尿道外口酵母菌携带种类较少,以光滑假丝酵母菌为主;女性尿道外口酵母菌携带率显著高于男性;部分酵母菌对常用抗真菌药物存在天然耐药现象。Objective:To investigate the yeast carrier rate,species distribution and susceptibility to common antifungal drugs in external urethral meatus of healthy college students.Methods:A total of 261 healthy university students were randomly recruited from a university in Yunnan Province,and the swabs from urinary meatus were collected for isolation,identification,drug sensitivity testing,and statistical analysis.Results:39 yeast species were isolated,with a carrier rate of 14.9%;among them,6 strains were isolated from 130 male samples,with a carrier rate of 4.6%,while 33 strains were isolated from 131 female samples,with a carrier rate of 25.2%.The difference of carrier rate between different gender groups was statiscally significant(χ2=21.73,P=0.005).Among 39 yeast species,there were 11 strains of Candida albicans(28.2%),24 strains of Candida glabrata(61.5%),4 strains of other Candida species(10.3%).The result showed that the geometric means of the yeast to fluconazole,flurocytosine and amphotericin B were 22.03,0.16 and 4.37μg/mL,respectively,and the drug resistance rates of isolates were 15.4%,0.0%and 100.0%,respectively.Conclusion:The prevalence of Candida colonization among healthy college students at urinary meatus is relatively low,with smooth colony Candida glabrata being the predominant species.The colonization rate of Candida among female students is significantly higher than that among male students.Some Candida strains exhibit intrinsic resistance to commonly used antifungal drugs.

关 键 词：健康大学生 酵母菌 携带率 尿道外口 药物敏感性 

分 类 号：R379.4[医药卫生—病原生物学]