人恶性胸膜间皮瘤组织中K-ras基因点突变的研究  

Research on the Point Mutation of K-ras Gene in Human Malignant Pleural Mesothelioma

作　　者：刘如爱 杨雪倩 王播勇 梅雯 周静华 熊伟 Liu Ruai;Yang Xueqian;Wang Boyong;Mei Wen;Zhou Jinghua;Xiong Wei(Pre-clinical College,Dali University,Dali,Yunnan 671000,China;Key Laboratory of Clinical Biochemistry in College and Universities of Yunnan Province,Dali,Yunnan 671000,China;Department of Pathology,The Fourth Affiliated Hospital of Dali University,Chuxiong,Yunnan 651000,China)

机构地区：[1]大理大学基础医学院,云南大理671000 [2]云南省高校临床生物化学检验重点实验室,云南大理671000 [3]大理大学第四附属医院病理科,云南楚雄651000

出　　处：《大理大学学报》2023年第4期43-49,共7页Journal of Dali University

基　　金：国家自然科学基金项目(82160516);云南省应用基础研究计划项目(202101AT070006);国家级大学生创新创业计划项目(202210679006);大理市科技计划项目(2021KBG032)。

摘　　要：目的:探究云南省大姚县青石棉污染区恶性胸膜间皮瘤(MPM)患者组织K-ras基因点突变。方法:应用聚合酶链式反应(PCR)-单链构象多态性分析和PCR-DNA测序技术联合检测59例MPM患者和13例非MPM患者胸膜间皮组织中K-ras基因密码子12、13和61的点突变情况。结果:59例MPM患者和13例非MPM患者胸膜间皮组织中均没有发生K-ras基因点突变,K-ras基因点突变的检出率为0.0%。结论:云南省大姚县青石棉污染区MPM患者组织K-ras基因突变型的比例较低,提示K-ras原癌基因点突变在此地区MPM的诱导发生中并不发挥关键作用。Objective:To investigate K-ras gene point mutation in malignant pleural mesothelioma(MPM)tissue of patients from the crocidolite-contaminated area in Dayao County,Yunnan Province.Methods:Polymerase chain reaction(PCR)and single-strand conformation polymorphism and PCR-DNA sequencing were used to detect the point mutations of K-ras codons 12,13 and 61 in pleural mesothelium of 59 MPM patients and 13 non-MPM patients.Results:There was no point mutation of K-ras gene in pleural mesothelial tissues of 59 MPM patients and 13 non-MPM patients,and the detection rate of K-ras gene point mutation was 0.0%.Conclusion:The proportion of K-ras gene mutation type in MPM tissue is relatively low,which suggests that K-ras proto-oncogene point mutation does not play a key role in the induction of MPM in the crocidolite-contaminated area of Dayao county,Yunnan Province.

关 键 词：恶性胸膜间皮瘤 K-RAS基因 点突变 聚合酶链式反应-单链构象多态性 

分 类 号：R735.9[医药卫生—肿瘤]