灭活菌体型后生元的体外降血压、降尿酸及降血糖作用  

Hypotensive,Hypouricemic,and Hypoglycemic Effect of Inactivated Bacterial Postbiotics in Vitro

作　　者：林倩如 邓怡玲 赵玥明 何泽琪 乔子骄 刘果[1] 石心茹 陈运娇[1] 肖苏尧[1] 侯艳梅 谢奎 曹庸[1] LIN Qianru;DENG Yiling;ZHAO Yueming;HE Zeqi;QIAO Zijiao;LIU Guo;SHI Xinru;CHEN Yunjiao;XIAO Suyao;HOU Yanmei;XIE Kui;CAO Yong(College of Food Science,South China Agricultural University,Guangzhou 510642,China;Hyproca Nutrition Co.Ltd.,Changsha 410011,China)

机构地区：[1]华南农业大学食品学院,广东广州510642 [2]海普诺凯营养品有限公司,湖南长沙410011

出　　处：《现代食品科技》2023年第4期19-25,共7页Modern Food Science and Technology

基　　金：国家自然科学基金项目(31972078);中国博士后科学基金面上项目(2020M672651)。

摘　　要：后生元对人体具有多种健康助益,是近年来研究的热点。某市售后生元是以大豆分离蛋白为主要原料开发制备的灭活菌体型后生元产品,该种原料类型的后生元产品鲜有报道。为了解其生物活性,研究采用大鼠胸大动脉平滑肌细胞(VSMC)、人肾小管上皮细胞(HK-2)、小鼠前脂肪细胞(3T3-L1)模型评价其体外降血压、降尿酸、降血糖作用。结果显示,该后生元产品能够显著抑制血管紧张素-Ⅱ(AngiotensinⅡ,AngⅡ)导致的VSMC细胞迁移及氧化应激,且具有浓度依赖性,作用质量浓度为200μg/mL时,迁移率降低12.44%,活性氧(Reactive Oxygen,ROS)释放量仅为空白组的86.51%。在HK-2细胞模型中,后生元能极显著降低HK-2细胞的尿酸含量,700μg/mL作用下,尿酸含量降低30.02%。而在降糖活性评价中,后生元无法促进胰岛素抵抗(Insulin Resistance,IR)的3T3-L1细胞吸收葡萄糖。研究结果表明,后生元具有显著的降血压、降尿酸生物活性,这为开发兼具降血压、降尿酸功能性食品或医药保健品提供了理论依据;细胞水平上,后生元不具有降血糖功效,可能需要动物模型进行验证。Postbiotics have various health benefits for the human body and have become a hot research topic in recent years.A commercially available,inactivated bacteriophage-type postbiotic product has been developed and prepared using soybean protein isolate as the main raw material.The scarcity of reports on such postbiotic products is the motivation for this study.To understand the biological activity of this postbiotic product,in this study,rat thoracic vascular smooth muscle cell(VSMC),human renal tubular epithelial cell(HK-2),and mouse preadipocyte(3T3-L1)models were used to evaluate its in vitro hypotensive,hypouricemic,and hypoglycemic properties.The results showed that the postbiotic product could significantly inhibit the migration and oxidative stress of VSMC cells induced by angiotensin II(AngⅡ)in a concentration-dependent manner,with a 12.44%reduction in migration rate at a concentration of 200μg/mL,and the release of reactive oxygen species(ROS)being 86.51%of that of the control group.In the HK-2 cell model,the postbiotic significantly reduced the uric acid content of HK-2 cells,with a 30.02%reduction at a concentration of 700μg/mL.In contrast,evaluation of hypoglycemic activity showed that the postbiotic was unable to promote glucose uptake by insulin-resistant(IR)3T3-L1 cells.The results of this study showed that the postbiotic has considerable hypotensive and hypouricemic biological activity,thus providing a theoretical basis for the development of functional foods or medical and health products with hypotensive and hypouricemic effects.At the cellular level,the postbiotic does not exhibit hypoglycemic effects,which need to be verified in animal models.

关 键 词：后生元 细胞模型 降血压 降尿酸 降血糖 

分 类 号：TS201.4[轻工技术与工程—食品科学]