Mg(OH)_(2)nanosheets on Ti with immunomodulatory function for orthopedic applications  

作　　者：Yue He Mengyu Yao Jielong Zhou Juning Xie Changxiang Liang Dong Yin Shuaihao Huang Yu Zhang Feng Peng Shi Cheng 

机构地区：[1]School of Medicine,South China University of Technology,Guangzhou 510006,China [2]Medical Research Center,Department of Orthopedics,Guangdong Provincial People’s Hospital,Guangdong Academy of Medical Sciences,Guangzhou 510080,China

出　　处：《Regenerative Biomaterials》2022年第1期325-334,共10页再生生物材料（英文版）

基　　金：supported by the National Natural Science Foundation of China(52001076);the Scientific and Technological Projects of Guangzhou,China(202102020431);the NSFC Incubation Project of Guangdong Provincial People’s Hospital(KY0120220044);the Guangdong Basic and Applied Basic Research Foundation(2021A1515110135).

摘　　要：Macrophages play a vital role for guiding the fate of osteogenesisrelated cells.It is well known that nano-topography and bioactive ions can directly enhance osteogenic behavior.However,the effects of nano-structure combined with bioactive ions release on macrophage polarization and the following osteogenesis and angiogenesis are rarely reported.Herein,Mg(OH)_(2)films with nano-sheet structures were constructed on the surface of Ti using hydrothermal treatment.The film presented nanosheet topography and sustained release of Mg ions.The results of in vitro culture of bone marrow-derived macrophages(BMDMs),including PCR,western blot and flow cytometry suggested that the nano-Mg(OH)_(2)films were more favorable for macrophages polarizing to tissue healing M2 phenotype.Moreover,air-pouch model confirmed that the nano-Mg(OH)_(2)film coated Ti would induce milder inflammation and thinner fibrous layer in vivo,compared with untreated Ti.Furthermore,macrophages-conditioned culture mediums were collected from nano-Mg(OH)_(2)coated Ti group was superior for the osteogenic behaviors of mice bone marrow stem cells and the angiogenic behaviors of human umbilical vein endothelial cells.With harmonious early inflammatory response and subsequently improved osteogenesis and angiogenesis,the nano-Mg(OH)_(2)coated Ti is promising for orthopedic applications.

关 键 词：IMMUNOMODULATION TITANIUM nano-magnesium hydroxide OSTEOGENESIS 

分 类 号：TB383[一般工业技术—材料科学与工程]