人参发酵菌种的筛选及皂苷转化能力研究  

Screening of ginseng fermented bacteria strains and research on transformation abilities of ginsenoside

作　　者：朱珺 钱永清 吴琴 崔竞文 林国栋 李瑞 李理 陈苏 ZHU Jun;QIAN Yongqing;WU Qin;CUI Jingwen;LIN Guodong;LI Rui;LI Li;CHEN Su(Hangzhou Wahaha Technology Co.Ltd.,Hangzhou 310018,China;Zhejiang Shaoxing Institute of Tianjin University,Shaoxing 312300,China)

机构地区：[1]杭州娃哈哈科技有限公司,浙江杭州310018 [2]天津大学,浙江绍兴研究院,浙江绍兴312300

出　　处：《食品与发酵工业》2023年第8期159-163,共5页Food and Fermentation Industries

基　　金：浙江省重点研发计划项目(2020C04002)。

摘　　要：以人参皂苷Rb 1、Rg 3转化情况为指标,从78株植物乳杆菌中筛选具有人参皂苷转化能力的菌株。考察优选菌株在人参提取液中的发酵特性以及多种皂苷转化情况,采用人结直肠肿瘤细胞模型考察菌株发酵液的抗肿瘤作用。结果显示,78株菌株中植物乳杆菌581表现最优,Rb 1转化率达到75.41%,Rg 3提高倍数达13.59。经581菌株发酵的人参提取液风味有所改善,在发酵16 h后,pH为3.64,活菌数为2.1×10^(8) CFU/mL。与人参提取液相比,发酵液中皂苷Rb 1、Rb 2、Rc、Rd、Re、Rg 1的含量显著降低(P<0.01),5种稀有皂苷(Rg 3、Rk 3、F 2、C-K、Rg 6)的含量则显著升高(P<0.01)。581菌株人参发酵液对肿瘤细胞的存活抑制率为(59.16±1.26)%,显著高于未发酵前的人参提取液(P<0.05)。结果表明,植物乳杆菌581具有良好的发酵特性,通过转化生成多种稀有人参皂苷,提高了人参产品的抗肿瘤活性,可用于发酵人参功能饮料的开发。The transformation rate of ginsenoside Rb 1 and Rg 3 were chosen as indicators to screen the potential ginseng fermented bacteria strains from 78 Lactiplantibacillus plantarum strains.After that,the fermenting properties in ginseng juice and the transformation abilities of multiple ginsenosides for the selected strain were detected.The anti-tumor effect of the fermented ginseng juice was determined using human colorectal tumor cells.The results showed that,among all the tested L.plantarum strains,L.plantarum 581 had the strongest ability to convert the ginsenoside.The Rb 1 conversion rate reached 75.41%and the Rg 3 level elevated 13.59 times during the fermentation of L.plantarum 581.Ginseng juice fermented by L.plantarum 581 had improved taste.After 16 hours′fermentation,pH dropped to 3.47 and the viable count of L.plantarum 581 reached 2.1×10^(8) CFU/mL.Compared to the ginseng extract,ginseng juice fermented by L.plantarum 581 had declined ginsenoside content of Rb 1,Rb 2,Rc,Rd,Re,and Rg 1(P<0.01),and elevated rare ginsenoside content of Rg 3,Rk 3,F 2,C-K and Rg 6(P<0.01).L.plantarum 581 fermented ginseng juice also showed significant elevated inhibition ratio of(59.16±1.26)%against tumor cells(P<0.05)in comparison to unfermented ginseng extract.The results indicated that L.plantarum 581 having good fermenting properties,can produce various rare ginsenosides through bio-transforming process and elevate the anti-tumor effects of ginseng products,which should be used for developing of ginseng fermented beverage with specific functions.

关 键 词：植物乳杆菌 人参 发酵 稀有人参皂苷 抗肿瘤 

分 类 号：TS275.4[轻工技术与工程—农产品加工及贮藏工程]