基于气相离子迁移谱技术的五倍子花中蜂蜜成熟度模型鉴别研究  

Study of maturity identification model for gallnut flower Apis cerana honey based on gas chromatography-ion mobility spectrometry

作　　者：刘振平[1] 甘芳瑗 刘国政[1] 庞钶靖 姜容[1] 聂青玉[2] 张艳[2] LIU Zhenping;GAN Fangyuan;LIU Guozheng;PANG Kejing;JIANG Rong;NIE Qingyu;ZHANG Yan(Chongqing Vocational Institute of Safety Technology,Chongqing 404020,China;Chongqing Three Gorges Vocational College,Chongqing 404155,China)

机构地区：[1]重庆安全技术职业学院,重庆404020 [2]重庆三峡职业学院,重庆404155

出　　处：《食品与发酵工业》2023年第8期265-271,共7页Food and Fermentation Industries

基　　金：重庆市教育委员会科学技术研究计划青年项目(KJQN201804703);重庆市教育委员会高校创新研究群体项目(CXQTP19037)。

摘　　要：采用气相离子迁移谱(gas chromatography-ion mobility spectrometry,GC-IMS)技术对重庆三峡库区5个不同成熟度的五倍子花中华蜜蜂蜂蜜(五倍子蜂蜜)的挥发性有机化合物(volatile organic compounds,VOCs)进行测定和分析。利用二维差谱法从广泛的VOCs信号数据中筛选出45个有效表征五倍子蜂蜜成熟度差异的特征变量,从差异化的VOCs分布轮廓的非靶向角度对5个不同成熟度的五倍子蜂蜜进行了偏最小二乘判别分析(partial least squares discriminant analysis,PLS-DA),并构建了五倍子蜂蜜成熟度鉴别模型。PLS-DA鉴别模型的拟合优度参数(R 2)和预测能力参数(Q 2)均较好,分别为0.880和0.706。交叉验证和置换模拟验证结果表明所构建模型未发生过拟合情况,模型稳健有效,可实现对5个不同成熟度的五倍子蜂蜜进行有效鉴别。同时,通过变量投影重要性(variable importance for the projection,VIP)值进一步筛选出了14个对有效区分五倍子蜂蜜成熟度发挥关键作用的特征标志物。该研究引入GC-IMS技术和PLS-DA的理论与方法实现了不同成熟度五倍子蜂蜜样品的准确鉴别,为蜂蜜质量控制提供了新的技术参考。The gas chromatography-ion mobility spectrometry(GC-IMS)was used to determine and analyze the volatile organic compounds(VOCs)in gallnut flower Apis cerana honey with 5 different maturity levels in Chongqing Three Gorges Reservoir Area.The 45 characteristic variables were selected from a wide range of VOCs signal data by the two-dimensional differential spectrum method.The partial least squares discriminant analysis(PLS-DA)was performed on gallnut flower Apis cerana honey with 5 different maturities from the untargeted perspective of differentiated VOCs distribution profile,and the maturity identification model of gallnut honey was established.The goodness of fit(R 2)and predictive power(Q 2)parameters of the PLS-DA discriminant model were both good,the values were 0.880 and 0.706,respectively.The results of cross-validation and permutation showed that the identification model did not overfit,and the identification model was robust and effective,which could effectively identify gallnut flower Apis cerana honey from 5 different maturities.At the same time,14 characteristic markers that played a key role in effectively distinguishing gallnut flower Apis cerana honey maturity were further screened out by variable importance for the projection(VIP)values.In this study,GC-IMS technology and PLS-DA theory and method were introduced to achieve accurate identification of gallnut flower Apis cerana honey from different maturities,which provided a new technical reference for the quality control of honey.

关 键 词：气相离子迁移谱 中华蜜蜂 蜂蜜成熟度 模型鉴别 五倍子蜂蜜 

分 类 号：S896.1[农业科学—特种经济动物饲养] O657[农业科学—畜牧兽医]