1-MCP处理采后红阳猕猴桃果实生理效应的研究  

Study on Physiological Effects of 1-MCP Treatment of Postharvest ′Hongyang′ Kiwifruit

作　　者：林晋雨 肖丽[1] 杨春平[1] 龚国淑[1] 陈华保[1] LIN Jinyu;XIAO Li;YANG Chunping;GONG Guoshu;CHEN Huabao(Sichuan Agricultural University,College of Agronomy,Chengdu 611130,China)

机构地区：[1]四川农业大学农学院,成都611130

出　　处：《四川农业大学学报》2023年第2期307-317,共11页Journal of Sichuan Agricultural University

基　　金：四川省重大科技专项(2017NZDZX0003)。

摘　　要：【目的】为探究1-甲基环丙烯(1-MCP)处理结合冷藏(1~4℃)贮藏采后‘红阳’猕猴桃果实条件下保鲜的分子调控机制。【方法】以‘红阳’猕猴桃果实为材料,采用1-MCP配合冷藏进行红阳猕猴桃保鲜,研究贮藏过程中果实生理效应的变化。【结果】1-MCP处理结合冷藏保藏可以减缓猕猴桃果实软化速度,抑制TSS、可滴定酸、VC及花色苷的降解,减缓MDA的积累,维持了SOD的活力,降低果实中乙烯释放量。利用RNA-Seq技术进行转录组学比较分析,共筛选获得2 380个差异表达基因,其中上调表达基因有1 503个,下调表达基因877个。GO富集分析表明,差异表达基因显著富集在细胞组分、分子功能和生物过程中的肽生物合成与代谢、酰胺生物合成、蛋白质运输、防御反应等功能类别。KEGG富集分析显示,上调基因主要富集于植物激素信号转导通路、半胱氨酸和蛋氨酸代谢通路以及苯丙氨酸代谢、谷胱甘肽代谢等抗氧化等相关通路,推测蛋氨酸代谢表达的增加是减少乙烯合成的主要原因之一。下调基因主要富集在丙酮酸代谢通路、叶绿素代谢、烟酸和烟酰胺代谢以及蛋白酶体代谢通路等影响呼吸作用强度的通路。【结论】1-MCP结合冷藏处理‘红阳’猕猴桃果实,可以通过提高果实抗氧化相关基因表达,减少乙烯合成前体,抑制呼吸相关基因表达等方式,达到延缓果实衰老而起到保鲜效果。研究结果为‘红阳’猕猴桃的保鲜研究提供理论依据。【Objective】 This study purposes to investigate the molecular regulation mechanism of preservation under the conditions of 1-MCP treatment combined with refrigeration(1-4 ℃) storage of postharvest 'Hong yang' kiwifruit.【Method】 In this experiment,the fruits were used as the material,and 1-MCP and refrigeration were used to observe the change in the physiological effects of fruits during storage.【Result】 The results showed that 1-MCP treatment combined with cold storage slowed down the decrease of kiwi fruit hardness,inhibited the degradation of TSS,titratable acid,VC and anthocyanin,slowed down the accumulation of MDA,maintained the vitality of SOD,and reduced the ethylene release to delay the arrival of the peak of ethylene release.By performing comparative transcriptomic analysis using RNA-seq technology,a total of 2 380 differentially expressed genes were screened,including 1 503upregulated and 877 downregulated expressed genes.The GO enrichment analysis indicated that the differentially expressed genes were significantly enriched in the functional categories of cellular components,molecular function,and biological processes,including peptide biosynthesis and metabolism,amide biosynthesis,protein transport,and defense response.KEGG enrichment analysis showed that the upregulated genes were mainly enriched in the plant hormone signaling transduction pathway,cysteine and methionine metabolism pathways,and antioxidant-related pathways such as phenylalanine metabolism and glutathione metabolism.It is speculated that the increased expression of methionine metabolism is one of the main reasons for reducing ethylene synthesis.The downregulation was mainly enriched in pyruvate metabolism pathways,chlorophyll metabolism,nicotinic niacin and nicotinamide metabolism,and proteasome metabolism pathway and other pathways that affect the intensity of respiration.【Conclusion】 1-MCP combined with cold treatment of 'Hong yang' kiwifruit,through improving the fruit antioxidantrelated gene expression,reduce the e

关 键 词：‘红阳’猕猴桃 采后 1-甲基环丙烯 冷藏 转录组学分析 

分 类 号：S663.4[农业科学—果树学]