白穗醋栗多酚鉴定及其抗氧化活性分析  

Identification of Polyphenols in White Currant Fruit and Their Antioxidant Activity

作　　者：霍俊伟 郭文荣[1,2] 乔锦莉 秦栋 刘红蕾 姚月英[1,2] 张妍 HUO Junwei;GUO Wenrong;QIAO Jinli;QIN Dong;LIU Honglei;YAO Yueying;ZHANG Yan(College of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin 150030,China;National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions,Harbin 150030,China;Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region),Ministry of Agriculture and Rural Affairs,Northeast Agricultural University,Harbin 150030,China;Heilongjiang Green Food Science Research Institute,Harbin 150030,China)

机构地区：[1]东北农业大学园艺园林学院,黑龙江哈尔滨150030 [2]寒地小浆果开发利用国家地方联合工程研究中心,黑龙江哈尔滨150030 [3]东北农业大学农业农村部东北地区园艺作物生物学与种质创制重点实验室,黑龙江哈尔滨150030 [4]黑龙江省绿色食品科学研究院,黑龙江哈尔滨150030

出　　处：《食品科学》2023年第8期219-227,共9页Food Science

基　　金：黑龙江省应用技术研究与开发计划项目(GA20B301,GA19B102);黑龙江省博士后特别资助项目(LBH-TZ2004);国家葡萄产业技术体系蓝靛果哈尔滨综合实验站项目(CARS-29-10)。

摘　　要：采用东北农业大学小浆果资源圃内白穗醋栗果实,利用固相萃取技术进行分离纯化得到多糖、花色苷、非花色苷等组分,测定各组分的化学成分,并进一步测定各组分的总酚以及总花色苷含量,使用1,1-二苯基-2-三硝基苯肼自由基、2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基、铁离子还原能力等方法测定其各组分抗氧化能力。结果表明:白穗醋栗果实中含有丰富的非花色苷多酚为78种,花色苷多酚只有一种为矢车菊素-3-葡萄糖苷,可以作为一种潜在的花青素提取原料;白穗醋栗果实多酚粗提液总酚含量为(34.93±0.47)mg/g;总花青素含量为(2.99±0.98)mg/g;果实表现出了较强的抗氧化活性。Crude polyphenols were extracted from white currant fruit from the small berry resource garden in Northeast Agricultural University and purified by solid phase extraction(SPE)into polysaccharide,anthocyanin and non-anthocyanin fractions.The chemical composition as well as the contents of total phenols and total anthocyanins in each fraction was determined.1,1-Diphenyl-2-picrylhydrazyl(DPPH)radical scavenging capacity and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)radical cation scavenging capacity,and ferric ion reducing antioxidant power(FRAP)were used to evaluate the antioxidant capacity of each fraction.The results showed that white currant fruit contained 78 non-anthocyanin polyphenols and only one anthocyanin polyphenol,corninin-3-glucoside,which can be used as a potential raw material for anthocyanin extraction.The contents of total phenols and total anthocyanins in the crude extract from white currant fruit were(34.93±0.47)and(2.99±0.98)mg/g,respectively.It had strong antioxidant capacity.

关 键 词：白穗醋栗 多酚表征 抗氧化活性 

分 类 号：S663.9[农业科学—果树学]