Cu-PAN@G-CS/PVA制备及在虾肉鲜度检测中的应用  

Preparation of Graphene-Incorporated Chitosan-Polyvinyl Alcohol Composite Film Loaded with Copper-1-(2-Pyridylazo)-2-naphthol Complex and Its Application to Monitor Shrimp Freshness

作　　者：郭亚平 陈琳珉 陈全胜 陈晓梅[1] GUO Yaping;CHEN Linmin;CHEN Quansheng;CHEN Xiaomei(College of Ocean Food and Biological Engineering,Jimei University,Xiamen 361021,China)

机构地区：[1]集美大学海洋食品与生物工程学院,福建厦门361021

出　　处：《食品科学》2023年第8期330-336,共7页Food Science

基　　金：福建省自然科学基金面上项目(2020J01677);国家自然科学基金面上项目(32072299)。

摘　　要：以1-(2-吡啶偶氮)-2-萘酚(1-(2-pyridylazo)-2-naphthol,PAN)合铜(Ⅱ)络合物(Cu-PAN)为H_(2)S指示剂,壳聚糖(chitosan,CS)/聚乙烯醇(polyvinyl alcohol,PVA)为膜基材料,石墨烯为增敏材料,采用流延法制备比色复合薄膜(Cu-PAN@G-CS/PVA),通过指示剂置换法,实现对虾肉中H_(2)S的检测。利用扫描电子显微镜和傅里叶红外光谱考察Cu-PAN@G-CS/PVA的形貌和官能团特征,通过紫外-可见吸收光谱考察鲜度膜对H_(2)S的响应性能。结果表明,随H_(2)S质量浓度增加,Cu-PAN@G-CS/PVA薄膜由紫红色变成黄色。利用Cu-PAN@G-CS/PVA薄膜监测明虾(Penaeus chinensis)在4℃和25℃贮藏的鲜度变化,色差变化值与总挥发性盐基氮含量有很好的相关性,可准确、快速指示虾肉的新鲜度,为水产品智能包装的开发提供参考。A colorimetric composite film(Cu-PAN@G-CS/PVA)for the detection of H_(2)S in shrimp meat by indicator displacement was prepared by tape casting method using copper(II)-1-(2-pyridylazo)-2-naphthol complex(Cu-PAN)as a H_(2)S-sensitive material,chitosan(CS)combined with polyvinyl alcohol(PVA)as a film-forming material and graphene as a sensitizing material.The morphology and functional groups of Cu-PAN@G-CS/PVA were investigated by scanning electron microscopy(SEM)and Fourier transform infrared spectroscopy(FTIR),and the sensing performance to H_(2)S was examined by ultraviolet-visible(UV-vis)absorption spectroscopy.Experimental results showed that the color of the Cu-PAN@G-CS/PVA film changed from purplish red to yellow with increasing H_(2)S concentration.This film was used to monitor the freshness of Penaeus chinensis stored at 4 or 25℃.It was shown that the trend of the color difference value(ΔE)of the Cu-PAN@G-CS/PVA film had a good correlation with the total volatile basic nitrogen(TVB-N)content of the sample.Therefore,the Cu-PAN@G-CS/PVA film could accurately and rapidly indicate the freshness of P.chinensis,which may provide a reference for the development of intelligent packaging of aquatic products.

关 键 词：1-(2-吡啶偶氮)-2-萘酚合铜(Ⅱ) 新鲜度 虾 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]