Quantitative proteomic and phosphoproteomic analyses of the hippocampus reveal the involvement of NMDAR1 signaling in repetitive mild traumatic brain injury  

作　　者：Zhicheng Tian Zixuan Cao Erwan Yang Juan Li Dan Liao Fei Wang Taozhi Wang Zhuoyuan Zhang Haofuzi Zhang Xiaofan Jiang Xin Li Peng Luo 

机构地区：[1]Department of Neurosurgery,Xijing Hospital,Fourth Military Medical University,Xi’an,Shaanxi Province,China [2]The Sixth Regiment,School of Basic Medicine,Fourth Military Medical University,Xi’an,Shaanxi Province,China [3]Department of Neurobiology,School of Basic Medicine,Fourth Military Medical University,Xi’an,Shaanxi Province,China [4]Medical Experiment Center,Shaanxi University of Chinese Medicine,Xianyang,Shaanxi Province,China [5]Department of Anesthesiology,The Second Hospital of Jilin University,Jilin University,Changchun,Jilin Province,China [6]School of Life Science,Northwest University,Xi’an,Shaanxi Province,China [7]Department of Anesthesiology,Xijing Hospital,Fourth Military Medical University,Xi’an,Shaanxi Province,China

出　　处：《Neural Regeneration Research》2023年第12期2711-2719,共9页中国神经再生研究（英文版）

基　　金：funded by the National Natural Science Foundation of China,Nos.82171363(to PL),82171321(to XL),82171458(to XJ);the Youth Nova Program of Shaanxi,No.2021KJXX-19(to PL)。

摘　　要：The cumulative damage caused by repetitive mild traumatic brain injury can cause long-term neurodegeneration leading to cognitive impairment.This cognitive impairment is thought to result specifically from damage to the hippocampus.In this study,we detected cognitive impairment in mice 6 weeks after repetitive mild traumatic brain injury using the novel object recognition test and the Morris water maze test.Immunofluorescence staining showed that p-tau expression was increased in the hippocampus after repetitive mild traumatic brain injury.Golgi staining showed a significant decrease in the total density of neuronal dendritic spines in the hippocampus,as well as in the density of mature dendritic spines.To investigate the specific molecular mechanisms underlying cognitive impairment due to hippocampal damage,we performed proteomic and phosphoproteomic analyses of the hippocampus with and without repetitive mild traumatic brain injury.The differentially expressed proteins were mainly enriched in inflammation,immunity,and coagulation,suggesting that non-neuronal cells are involved in the pathological changes that occur in the hippocampus in the chronic stage after repetitive mild traumatic brain injury.In contrast,differentially expressed phosphorylated proteins were mainly enriched in pathways related to neuronal function and structure,which is more consistent with neurodegeneration.We identified N-methyl-D-aspartate receptor 1 as a hub molecule involved in the response to repetitive mild traumatic brain injury,and western blotting showed that,while N-methyl-D-aspartate receptor 1 expression was not altered in the hippocampus after repetitive mild traumatic brain injury,its phosphorylation level was significantly increased,which is consistent with the omics results.Administration of GRP78608,an N-methyl-D-aspartate receptor 1 antagonist,to the hippocampus markedly improved repetitive mild traumatic brain injury-induced cognitive impairment.In conclusion,our findings suggest that N-methyl-D-aspartate receptor 1 sig

关 键 词：cognitive impairment Grin1 HIPPOCAMPUS learning memory N-METHYL-D-ASPARTATE N-methyl-D-aspartate receptor 1 phosphoproteomic PROTEOMIC repetitive mild traumatic brain injury(rmTBI) secondary injury 

分 类 号：R651.15[医药卫生—外科学]