鹰嘴豆慢生根瘤菌USDA 3378发酵条件优化  

Optimization of fermentation conditions of chickpea rhizobia Mesorhizobium ciceri USDA 3378

作　　者：张俊杰 王楠 郭晨 王京琪 冯煜锋 ZHANG Junjie;WANG Nan;GUO Chen;WANG Jingqi;FENG Yufeng(School of Food and Bioengineering,Zhengzhou University of Light Industry,Zhengzhou 450002,China)

机构地区：[1]郑州轻工业大学食品与生物工程学院,河南郑州450002

出　　处：《中国酿造》2023年第4期210-214,共5页China Brewing

基　　金：国家自然科学基金项目(31970006);河南省高校科技创新人才项目(22HASTIT035);河南省青年人才托举工程项目(豫科协发[2019]2号)。

摘　　要：该研究通过比较中慢生根瘤菌(Mesorhizobium ciceri)USDA 3378在酵母甘露醇琼脂(YMA)、酵母胰蛋白胨(TY)及修正YMA(MYMA)培养基中的生长情况,初步筛选最佳培养基。采用单因素试验及Box-Behnken响应面试验优化菌株USDA 3378发酵条件,并通过发酵罐扩大培养评价菌株USDA 3378的生长情况。结果表明,Mesorhizobium ciceri USDA 3378初筛培养基为YMA,其最佳发酵条件为甘露醇12 g/L,酵母粉8.75 g/L,KH2PO40.25 g/L,K2HPO40.25 g/L,无水Mg SO40.1 g/L,Na Cl 0.l g/L,接种量4%(V/V)。在此优化条件下,OD600 nm值为2.208,比优化前提高了35.54%。菌株USDA 3378在3 L发酵罐扩大培养后,OD600 nm值为2.341,比优化前提高了10.42%。The optimal medium was primarily selected through comparing the growth of Mesorhizobium ciceri USDA 3378 in yeast mannitol agar(YMA),tryptone yeast(TY)and modified YMA(MYMA)medium.The fermentation conditions were optimized by single factor experiments and Box-Behnken response surface methodology,and the growth of strain USDA 3378 were evaluated by fermenter culture.The results showed that the primarily selected medium was YMA,the optimal fermentation conditions were as follows:mannitol 12 g/L,yeast powder 8.75 g/L,KH2PO40.25 g/L,K2HPO40.25 g/L,anhydrous MgSO40.1 g/L,NaCl 0.1 g/L,and inoculum 4%(V/V).Under these optimal conditions,the OD600 nm value was 2.208,which was 35.54%higher than that before optimization.The OD600 nm value could reach 2.341 after expanded culture in the fermenter,which was 10.42%higher than that before optimization.

关 键 词：鹰嘴豆 慢生根瘤菌 培养基 发酵条件优化 响应面法 

分 类 号：S144.3[农业科学—肥料学]