木姜子新鲜果实挥发油成分分析及抗氧化活性研究  

Analysis of volatile oil constituents in fresh fruit of Litsea pungens Hemsl.and its antioxidant activity

作　　者：张建 黄艳 刘秋妍 赵麟 尤霜 谢天芳 代倩 饶朝龙 ZHANG Jian;HUANG Yan;LIU Qiuyan;ZHAO Lin;YOU Shuang;XIE Tianfang;DAI Qian;RAO Chaolong(School of Public Health,Chengdu University of Traditional Chinese Medicine,Chengdu 611137;R&D Center for Efficiency,Safety and Application in Chinese Materia Medica with Medical and Edible Values,School of Public Health,Chengdu University of Traditional Chinese Medicine,Chengdu 611137;Yaomazi Food Co.,Ltd.,Meishan 620000)

机构地区：[1]成都中医药大学公共卫生学院,成都611137 [2]成都中医药大学公共卫生学院药食同用中药效用与安全研究中心,成都611137 [3]幺麻子食品股份有限公司,眉山620000

出　　处：《中国食品添加剂》2023年第4期269-275,共7页China Food Additives

基　　金：中央引导地方科技发展专项(2020ZYD058);成都中医药大学学科人才科研提升计划(XSGG2019011、MPRC2021045)。

摘　　要：目的:明确川产木姜子新鲜果实挥发油的主要成分及其抗氧化活性,为木姜子的开发利用提供理论依据。方法:利用单因素试验和正交试验优化水蒸气蒸馏法挥发油的提取工艺,通过GC-MS检测挥发油中的主要成分,采用羟自由基(·OH)和1,1-二苯基-2-三硝基苯肼(DPPH·)清除试验测定其抗氧化活性。结果:木姜子鲜果挥发油得率在加热时间6 h,料液比1∶20 g/mL的条件下达到最优,其得率为4.59%。GC-MS分析木姜子鲜果挥发油中的主要成分为柠檬醛、芳樟醇和柠檬烯。抗氧化试验测得其·OH和DPPH·清除能力的IC_(50)分别为19.650 mg/mL和18.994 mg/mL。结论:优化后木姜子鲜果挥发油的提取工艺稳定、可靠,通过GC-MS分析发现木姜子鲜果挥发油中含有丰富的芳香化合物和抗氧化物质,说明木姜子可以作为天然食品添加剂的原料。Objective:To clarify the main components and antioxidant activity in the volatile oil of fresh Litsea pungens Hemsl.produced in Sichuan,and to provide a theoretical basis for the development and utilization of Litsea pungens Hemsl.Methods:The extraction conditions of volatile oil with steam distillation were optimized by single factor experiment and orthogonal experiment.The main components in the volatile oil were detected by GC-MS.The hydroxyl radical(·OH)and 1,1-diphenyl-2-picrylhydrazyl(DPPH·)scavenging experiments were used to determine its antioxidant activity.Results:The optimal yield of volatile oil was 4.59%when the heating time was 6 h and the ratio of solid to liquid was 1∶20 g/mL.The main components of the volatile oil were citral,linalool and limonene.The IC_(50) of its·OH and DPPH·scavenging abilities were measured as 19.650 mg/mL and 18.994 mg/mL,respectively.Conclusion:With the optimized conditions,the extraction process of the volatile oil of the Litsea pungens Hemsl.was stable and reliable.The GC-MS analysis showed that the volatile oil of Litsea pungens Hemsl.was rich in aromatic compounds and antioxidants,indicating that Litsea pungens Hemsl.can be used as a raw material for natural food additives.

关 键 词：木姜子 挥发油 成分分析 抗氧化 

分 类 号：TS202.3[轻工技术与工程—食品科学]