济南市零售鸡肉中沙门菌污染状况及可移动黏菌素耐药基因分析  

Contamination and mobile colistin resistance gene analysis for Salmonella isolated from retail chickens in Ji’nan

作　　者：刘畅 李楠 胡豫杰 阚浩鹏 杨柱 李凤琴 温红玲 赵丽 LIU Chang;LI Nan;HU Yujie;KAN Haopeng;YANG Zhu;LI Fengqin;WEN Hongling;ZHAO Li(Department of Microbiological Laboratory Technology,School of Public Health,Cheeloo College of Medicine,Shandong University,Key Laboratory of Infectious Disease Control and Prevention in Universities of Shandong,Shandong Ji’nan 250012,China;Key Laboratory of Food Safety Assessment of Ministry of Health,China National Center for Food Safety Assessment,Beijing 100021,China)

机构地区：[1]山东大学齐鲁医学院公共卫生学院微生物检验学系山东省“十三五”高等学校感染性疾病防控重点实验室,山东济南250012 [2]国家食品安全风险评估中心国家卫生健康委员会食品安全风险评估重点实验室,北京100021

出　　处：《中国食品卫生杂志》2023年第2期283-288,共6页Chinese Journal of Food Hygiene

摘　　要：目的了解2020—2021年济南市零售鸡肉中沙门菌的污染状况,并探究可移动黏菌素耐药基因(mcr)的携带情况。方法2020年12月至2021年11月在济南市采集零售鸡肉样品260份,依据GB 4789.4—2016《食品安全国家标准食品微生物学检验沙门氏菌检验》对样品中的沙门菌进行分离鉴定,通过聚合酶链式反应(PCR)对分离株进行血清型鉴定和mcr基因筛选,使用微量肉汤法对mcr基因阳性株开展抗生素敏感性试验。结果260份零售鸡肉样品中共检出阳性样品61份,污染率为23.46%(61/260),秋季污染率最高可达53.33%(32/60);共分离出沙门菌103株,56株为肠炎沙门菌,占比54.37%(56/103)。2株不同产地鸡翅样品来源的印第安纳沙门菌分离株检测出mcr-1基因,阳性率为1.94%(2/103)。2株mcr-1基因阳性印第安纳沙门菌分离株均为多重耐药株,其中1株可对碳青霉烯类和多黏菌素类在内的全部12类测试抗生素同时耐药。结论2020—2021年济南市零售鸡肉中的沙门菌污染较为严重,秋季采集样品的污染率较高,肠炎沙门菌为优势血清型,检出携带mcr-1基因;同时耐受黏菌素和碳青霉烯类抗生素的严重多重耐药沙门菌,应引起关注,并加强对鸡肉生产全链条中沙门菌的持续监测,为防控沙门菌引起的食源性疾病提供重要的基础数据。Objective To investigate the contamination of Salmonella in retail chickens in Ji’nan city from 2020 to 2021,and to explore the prevalence of the mobile colistin resistance gene(mcr)among these Salmonella isolates.Methods From December 2020 to November 2021,260 retail chicken samples were collected in Ji’nan City,and Salmonella was isolated and confirmed according to GB 4789.4—2016“National food standard-Food microbiological examination:Salmonella”.Serotype identification and mcr gene screening were performed for all Salmonella isolates by polymerase chain reaction(PCR).The drug resistance of mcr gene-positive strains was tested by micro-broth dilution method.Results A total of 61 in 260 retail chicken samples were positive for Salmonella detection with the contamination rate of 23.46%(61/260).The highest contamination rate was 53.33%(32/60)for samples collected in autumn.One hundred and three Salmonella strains were isolated,56 of which were Salmonella Enteritidis,accounting for 54.37%(56/103).Two Salmonella Indiana,isolated from two separate chicken wing samples collected from different regions,were positive for mcr-1 gene,with a positive rate of 1.94%(2/103).Antimicrobial susceptibility testing revealed that both of the two mcr-1-harboring Salmonella Indiana were multi-drug resistant,and one of them was concurrently resistant to all 12 categories of tested drugs,including carbapenems and polymyxins.Conclusion There was a certain degree of Salmonella contamination in retail chickens in Jinan from 2020 to 2021,and the contamination rate of the samples collected in autumn was higher than in any other seasons.Salmonella Enteritidis was the predominant serotype.It should be of concern that a Salmonella isolate carried mcr-1 gene with severe multidrug resistance to both colistin and carbapenems,indicating that the surveillance for Salmonella in the whole chain of chicken production should be strengthened continuously,to provide important basic data for the prevention and control of foodborne diseases.

关 键 词：济南市 鸡肉 沙门菌 mcr基因 耐药 

分 类 号：R155[医药卫生—营养与食品卫生学]