茶黄素(TF_(1))对人肝癌Bel-7402细胞的抑制作用及机制研究  

Inhibitory Effect and Mechanism of Theaflavin(TF_(1))on Hepatoma Carcinoma Bel-7402 Cells

作　　者：王专 韦武均 任珍珍 何志龙 范玉纯 周洁 郭桂义[4] 蒋利和 WANG Zhuan;WEI Wujun;REN Zhenzhen;HE Zhilong;FAN Yuchun;ZHOU Jie;GUO Guiyi;JIANG Lihe(College of Light Industry and Food Engineering,Guangxi University,Nanning 530004,China;School of Basic Medical Sciences,Youjiang Medical University for Nationalities,Baise 533000,China;Medical College,Guangxi University,Nanning 530004,China;Henan Key Laboratory of Tea Comprehensive Utilization in South Henan Xinyang Agriculture and Forestry University,Xinyang 464000,China)

机构地区：[1]广西大学轻工与食品工程学院,广西南宁530004 [2]右江民族医学院基础医学院,广西百色533000 [3]广西大学医学院,广西南宁530004 [4]信阳农林学院河南茶叶综合利用重点实验室,河南信阳464000

出　　处：《茶叶科学》2023年第2期287-296,共10页Journal of Tea Science

基　　金：南宁市青秀区重点研发项目(2020023);右江民族医学院高层次引进人才项目(YY2021sk02);河南豫南茶叶综合利用重点实验室开放基金(HNKLTOF2018005);福建省生态产业绿色技术重点实验室开放资金(WYKF2020-5)。

摘　　要：探究茶黄素(TF_(1))对人肝癌Bel-7402细胞的影响,并阐明其作用机制。通过CCK-8检测细胞活力,用平板克隆形成试验检测细胞增殖能力,细胞划痕愈合试验和Transwell小室法检测细胞迁移,用流式细胞术检测细胞凋亡,Western blot检测细胞凋亡相关蛋白(Bax、Bcl-2、PARP)、迁移相关蛋白(E-cad、N-cad、Vimentin、MMP9)和信号通路相关蛋白(TGF-β、smad3、p-smad3)表达水平。结果表明,不同剂量TF_(1)均可抑制Bel-7402细胞的增殖和迁移,促进其凋亡,并且存在剂量依赖性,剂量越大,抑制作用越强(P<0.05)。与对照组相比,试验组Bax、E-cad蛋白表达水平较高,Bcl-2、PARP,N-cad、Vimentin、MMP9、TGF-β、smad3、p-smad3表达水平较低(P<0.05)。茶黄素可能通过TGF-β信号通路抑制肝癌Bel-7402细胞的增殖、迁移,且促进其凋亡。To explore the inhibitory effect and mechanism of theaflavin(TF_(1)),human hepatoma carcinoma Bel-7402 cells were treated with different concentrations of TF_(1).Cell viability was detected by CCK8 and cell proliferation was detected by colony formation assay.Cell migration was detected by cell wound healing experiment and transwell chamber assay.Cell apoptosis was detected by flow cytometry.Apoptosis-related proteins(Bax,Bcl-2,PARP),migration related proteins(E-cad,N-cad,Vimentin,MMP9)and signaling pathway related proteins(TGF-β,Smad3,p-smad3)were detected by western blot.The results show that different concentrations of TF_(1) could inhibit the proliferation and migration of Bel-7402 cells and promote their apoptosis in a dose-dependent manner.The higher the dose,the stronger the inhibition effect(P<0.05).Compared with the control group,the expression levels of Bax and E-cad proteins in the experimental group were higher,while the expression levels of Bcl-2,PARP,N-cad,Vimentin,MMP9,TGF-β,Smad3 and p-smad3 were lower(P<0.05).In conclusion,TF_(1) could inhibit the proliferation and migration of Bel-7402 cells and promote their apoptosis through TGF-βsignaling pathway.

关 键 词：茶黄素 人肝癌细胞 机制研究 

分 类 号：S517.1[农业科学—作物学] R735.2[医药卫生—肿瘤]