正丁醇-盐酸法测定花生红衣中原花青素的方法优化  

Optimization of n-Butyl Alcohol-Hydrochloric Acid Method for Determination of Proanthocyanidins in Peanut Testa

作　　者：何晓勇 王璇 郭楠楠[1,2] 尚雪可 HE Xiao-yong;WANG Xuan;GUO Nan-nan;SHANG Xue-ke(College of Food Science and Engineering,Zhengzhou University of Science and Technology,Zhengzhou 450064,China;Zhengzhou City Food Safety Rapid Test Key Laboratory,Zhengzhou University of Science and Technology,Zhengzhou 450064,China)

机构地区：[1]郑州科技学院食品科学与工程学院,河南郑州450064 [2]郑州科技学院郑州市食品安全快速检测重点实验室,河南郑州450064

出　　处：《化学试剂》2023年第5期162-167,共6页Chemical Reagents

基　　金：河南省高等学校重点科研项目(21B550008)。

摘　　要：以花生红衣为原料,以原花青素含量为指标,采用优化后的正丁醇-盐酸法测定花生红衣提取物中原花青素的含量。通过单因素试验确定液料比、烘箱温度、保温时间对原花青素含量的影响。采用响应面软件,以单因素试验为基础,设计三因素三水平的响应面试验,并对试验结果进行分析。结果表明,影响花生红衣中原花青素含量的因素顺序为液料比>烘箱温度>保温时间,根据实际试验可行性,优化后的正丁醇-盐酸法的最佳条件分别为液料比65∶1(mL/g)、烘箱温度90℃、保温时间60 min,在此条件下原花青素的含量达到最高为135.003 mg/g。该方法的加标回收率为97.48%~98.78%,相对标准偏差为0.4%~1.1%。优化后的正丁醇-盐酸法具有较高的准确度和精密度,可为原花青素含量的测定方法优化提供参考。Using peanut red coat as raw material and proanthocyanidin content as index,the content of proanthocyanidins in peanut testa was determined by optimized n-butyl alcohol-hydrochloric acid method.The effects of liquid feed ratio,oven temperature and oven time on the content of procyanidins were determined by a single factor test.Using response surface software,based on single factor,three factor and three level response surface tests were designed,and the test results were analyzed.The results showed that the factors affecting the proanthocyanidins content in peanut testa were liquid-material ratio>oven temperature>holding time.According to the actual test situation,the optimum conditions of the optimized n-butyl alcohol-hydrochloric acid method were material ratio liquid 65∶1(mL/g),oven temperature 90℃and oven time 60 min.Under these conditions,proanthocyanidins reached a maximum content of 135.003 mg/g.The standard addition recovery of this method was 97.48%to 98.78%,and the relative standard deviation was 0.4%~1.1%.The optimized n-butanol-hydrochloric acid method has high accuracy and precision,which can provide reference for the optimization of method of determination procyanidins content.

关 键 词：花生红衣 原花青素 正丁醇-盐酸法 方法优化 

分 类 号：O657.32[理学—分析化学]