窖泥产氨菌的分离筛选与发酵培养基的优化  

Isolation and Screening of Ammonia Producing Bacteria from Pit Mud and Optimization of Fermentation Medium

作　　者：陈园 王超凯 李觅 覃超 刘念 明红梅 CHEN Yuan;WANG Chaokai;LI Mi;QIN Chao;LIU Nian;MING Hongmei(College of Bioengineering,Sichuan University of Science and Engineering,Yibin Sichuan 644005,China;Sichuan Food and Fermentation Industry Research&Design Institute Co.,Ltd.,Chengdu Sichuan 611130,China;Key Laboratory of Brewing Engineering and Application of China National Light Industry Council,Chengdu Sichuan 611130,China;Liu Nian’s Master Studio of Oenological Skills,Chengdu Sichuan 611130,China)

机构地区：[1]四川轻化工大学生物工程学院,四川宜宾644005 [2]四川省食品发酵工业研究设计院有限公司,四川成都611130 [3]中国轻工业酿酒工程及应用重点实验室,四川成都611130 [4]刘念酿酒师技能大师工作室,四川成都611130

出　　处：《食品与发酵科技》2023年第2期43-49,共7页Food and Fermentation Science & Technology

基　　金：兵团财政科技计划项目(2021AB005);四川省科技厅成果转移转化示范项目(2021ZHCG0080),四川省科技厅重点研发项目(2023YFS0477);泸州市科技局重点研发项目(2022-XDY-189)。

摘　　要：为获取可提升窖泥pH的高产氨态氮菌株,本文以优质老窖泥为菌源,采用多次富集培养的方式,从中筛选到一株高产氨态氮的菌株J19。对菌株进行形态学观察和分子生物学鉴定;并采用正交设计对氨态氮发酵培养基组分进行优化。结果表明,菌株J19为陆地寡养单胞菌(Stenotrophomonas terrae);发酵培养最佳组分为蛋白胨5.0 g/L,K2HPO4·3H2O 0.20 g/L,MgSO4·7H2O 0.50 g/L,FeSO4·7H2O 0.010 g/L,氨态氮产量为232.176 mg/L。In order to obtain a high ammonium nitrogen-producing strain that can raise the pH of cellar mud,this paper used high quality old cellar mud as the source of bacteria,and used multiple enrichment culture,from which a high ammonium nitrogen-producing strain J19 was selected.Morphological observation and molecular biology identification of the strains.An orthogonal design was also used to optimize the ammonium nitrogen fermentation medium components.The results showed that strain J19 was Stenotrophomonas terrae,The best medium was peptone 5.0g/L,K2HPO4·3H2O 0.20g/L,MgSO4·7H2O 0.50g/L,FeSO4·7H2O 0.010g/L,and the ammonia nitrogen content was 232.176mg/L.

关 键 词：窖泥 氨化细菌 寡养单胞菌属 富集筛选 正交设计 

分 类 号：TS261.13[轻工技术与工程—发酵工程]