便携式水产品四环素含量检测装置研制  

Development of a portable device for the detection of tetracycline contents in the aquatic products

作　　者：刘倩[1,2,3] 张航 蒋开拓[1,2] 黄国平 由天艳 LIU Qian;ZHANG Hang;JIANG Kaituo;HUANG Guoping;YOU Tianyan(School of Agricultural Engineering,Jiangsu University,Zhenjiang 212013,China;Key Laboratory of Modern Agricultural Equipment and Technology,Ministry of Education,Jiangsu University,Zhenjiang 212013,China;CECEP Solar Energy Technology(Zhenjiang)Co.,Ltd.,Zhenjiang 212013,China)

机构地区：[1]江苏大学农业工程学院,镇江212013 [2]江苏大学现代农业装备与技术教育部重点实验室,镇江212013 [3]中节能太阳能科技(镇江)有限公司,镇江212013

出　　处：《农业工程学报》2023年第5期8-14,共7页Transactions of the Chinese Society of Agricultural Engineering

基　　金：江苏省优势学科项目(PAPD-2018-87);中国博士后科学基金项目(2022M713499)。

摘　　要：针对水产品四环素残留的便捷化精准检测难题,该研究研制了一种四环素含量检测的便携式直读光电化学装置,并探究了装置用于鱼肉四环素残留检测的可行性。装置分别以LED和STC89C52单片机为激发光源和控制核心,利用设计的光源暗室避免外源光照对检测结果的影响。测试结果表明,LED激发光源的输出信号稳定,装置对于光电流信号的采集具有较好的准确性。进一步基于丝网印刷电极构建光电化学适配体传感器,采用所研制装置对信号进行采集与处理,最终以OLED显示器直接读取抗生素浓度信息。该方法对于四环素检测的线性范围为1×10-11~1×10-8mol/L,检测限为3×10-12mol/L,具有较好的选择性,且对于鱼肉四环素残留检测的回收率为92%~94%,为水产品四环素的精准现场分析提供了新的方法与途径。Development of efficient and reliable detection methods for sensitive detection of tetracycline residues is of great significance to ensure the foods quality and human health.Due to the lack of accuracy and convenience of the existing methods in detection of tetracycline residue,a portable photoelectrochemical(PEC)device for detection of tetracycline was designed and developed in this work,and the feasibility of the developed device to detect tetracycline residues in fish was further explored.For the development of the portable device,light emitting diode(LED)and STC89C52 microcontroller were employed as excitation light source and control core,respectively.In order to avoid the interference of external light on the detection results of the LED activated PEC sensor,a darkroom of light source was designed for the detection device,which was obtained by using 3D printing method.Then the output signal of the light source and the accuracy for the current signal collection of the developed device was studied.The results showed that the output signal of LED excitation light source was stable,and by comparison,the signal collected by the developed portable device was basically consistent with the commercial CHI660E electrochemical workstation used in the laboratory,which proved that the developed detection device has good accuracy for the collection of current signal and can be further used.For the construction of the PEC sensor,an integrated screen printing electrode was used to replace the traditional three-electrode system to further simplify the detection device,Bi2S3/g-C3N4 was used as photoactive material for the construction of the sensing interface and aptamer was used as the specific recognition element for selective recognition of target tetracycline.By the combination of the developed portable device and the proposed PEC sensor,a simple method for sensitive and on-site detection of tetracycline was finally fabricated.The current of the PEC sensor increased gradually with the increase of the tetracycline concen

关 键 词：抗生素 检测 水产品 电化学传感器 四环素 

分 类 号：S24[农业科学—农业电气化与自动化]