不同地区鲊广椒细菌多样性比较研究  

Comparative Study on Bacterial Diversity of Zha-Chili in Different Regions

作　　者：杨晨 柯悦 黄锶钘 刘梦琦 张彦 郭壮[1,2] 王玉荣[1,2] YANG Chen;KE Yue;HUANG Sixing;LIU Mengqi;ZHANG Yan;GUO Zhuang;WANG Yurong(Hubei Provincial Engineering and Technology Research Center for Food Ingredients,Hubei University of Arts and Science,Xiangyang 441053,China;Xiangyang Lactic Acid Bacteria Biotechnology and Engineering Key Laboratory,Hubei University of Arts and Science,Xiangyang 441053,China;Hubei Provincial Key Laboratory of Yeast Function,Angel Nutritech Co.,Ltd.,Yichang 443003,China)

机构地区：[1]湖北文理学院,湖北省食品配料工程技术研究中心,湖北襄阳441053 [2]湖北文理学院,乳酸菌生物技术与工程襄阳市重点实验室,湖北襄阳441053 [3]安琪纽特股份有限公司,酵母功能湖北省重点实验室,湖北宜昌443003

出　　处：《食品工业科技》2023年第10期145-150,共6页Science and Technology of Food Industry

基　　金：襄阳市基础研究类科技计划项目(2021ABS002937);湖北文理学院大学生创新创业训练计划项目(X202210519191);湖北省自然科学基金计划联合基金项目(2022CFD008)。

摘　　要：本研究使用IlluminaMiSeq测序技术解析兴山和保靖鲊广椒细菌群落结构。α多样性分析表明,兴山鲊广椒群落多样性及丰度均低于保靖鲊广椒(P<0.05)。两地鲊广椒中细菌主要是隶属于厚壁菌门(Firmicutes)的Lactobacillus(乳酸杆菌属),其在兴山鲊广椒中含量为50.24%,在保靖鲊广椒中为88.26%。β多样性分析发现,兴山和保靖鲊广椒细菌群落结构整体上具有显著差异(P<0.01),同时发现11个差异显著的细菌类群,其中得分较高的为Lactobacillus和Chishuiella(赤水河菌属)。表型预测发现,兴山鲊广椒细菌类群在可移动原件、生物膜形成、革兰氏阴性、致病潜力和氧化胁迫耐受等表型上表达显著,而保靖鲊广椒细菌类群则在革兰氏阳性表型上表达显著。In this study,Illumina MiSeq sequencing technology was used to analyze the bacterial community structure of Zha-Chili from Xingshan and Baojing.Theα-diversity analysis showed that the community diversity and abundance of Zha-Chili in Xingshan were significantly lower than those in Baojing(P<0.05).Lactobacillus belonging to the Firmicutes was the main bacteria in the two places,the abundance of which was 50.24%in Xingshan and 88.26%in Baojing.βdiversity analysis found that the bacterial community structure of Xingshan and Baojing Zha-Chili was significantly different on the whole(P<0.01)and 11 biomarkers were identified,among which Lactobacillus and Chishuiella were the most influential ones.Phenotypes prediction showed that bacterial groups of Zha-Chili of Xingshan were significantly expressed in movable elements,biofilm formation,gram negative,pathogenic potential and tolerance to oxidative stress,while the bacteria in Baojing Zha-Chili were significantly expressed in gram-positive.

关 键 词：高通量测序 鲊广椒 群落结构 细菌多样性 表型预测 

分 类 号：TS213.4[轻工技术与工程—粮食、油脂及植物蛋白工程]