重组人源胶原蛋白对D-半乳糖致衰老小鼠的抗衰老作用及机制研究  

Anti-aging Effects and Mechanisms of Recombinant Human-derived Collagen on Aging Mouse Induced by D-Galactose

作　　者：李佳 安苗青 吕晨豪 郭荣香 杜冰[2] 贺庆 LI Jia;AN Miaoqing;LÜChenhao;GUO Rongxiang;DU Bing;HE Qing(Jiangsu Aland Nutrition Co.,Ltd.,Taizhou 214500,China;College of Food Science,South China Agricultural University,Guangzhou 510000,China)

机构地区：[1]江苏艾兰得营养品有限公司,江苏泰州214500 [2]华南农业大学食品学院,广东广州510000

出　　处：《食品工业科技》2023年第10期343-352,共10页Science and Technology of Food Industry

基　　金：广东省自然科学基金面上项目(2020A1515011268)。

摘　　要：为探究重组人源胶原蛋白(recombinant human-derived collagen,RHC)的抗衰老作用及机制,通过300 mg/kg D-半乳糖腹腔注射构建衰老小鼠模型,并采用不同剂量RHC进行干预,研究衰老模型小鼠皮肤透明质酸、胶原蛋白、脂褐素、羟脯氨酸含量、抗氧化酶活性、与表皮细胞增殖分化和胶原蛋白合成相关的关键基因、蛋白的表达量等变化。结果表明,与衰老模型组(SLM)相比,小鼠经过RHC干预后,皮肤的褶皱程度明显减轻,胶原蛋白含量和含水量显著增加(P<0.05),超氧化物歧化酶(Superoxide dismutase,SOD)含量增加,丙二醛(Malondialdehyde,MDA)含量显著减少(P<0.0001)。与SLM组相比,RHC处理组的抗氧化指标,如谷胱甘肽过氧化物酶(GSH-Px)含量显著增加,而褐质素含量、病理评分均有所降低,且不同剂量处理组之间也有一定的差异性。此外,RHC处理组小鼠皮肤中的关键基因(EGFR、TIMP-1、COL1A2、COL3A1、TGF-βⅠ、TGF-βⅡ)的表达水平呈上升趋势,MMP-1、MMP-3基因和蛋白的表达降低。综上所述,RHC具有良好的抗皮肤衰老作用,其作用机制可能通过抗氧化、以及改善EGFR、TGF信号通路,调节MMPs和TIMPs动态平衡,促进表皮细胞的增殖分化和胶原蛋白合成从而起到延缓皮肤衰老的作用。In order to explore the anti-aging effect and mechanism of recombinant recombinant human-derived collagen(RHC),the aging mouse model was constructed through 300 mg/kg D-galactose intraperitoneal injection,and different doses of RHC were used to study the changes of hyaluronic acid,collagen,lipofuscin,hydroxyproline content,antioxidant enzyme activity,key genes and protein expression related to epidermal cell proliferation and differentiation and collagen synthesis.The results showed that compared with the aging model group(SLM),the intervention groups that was gavaged RHC had significantly less skin folds,higher collagen content(P<0.05)and superoxide dismutase(SOD),while lower malondialdehyde(MDA)content in the skins(P<0.0001).Compared with the SLM group,antioxidant indicators such as glutathione peroxidase(GSH-Px)content were increased in the RHC intervention groups,while lipofuscin content and pathological scores were reduced,and significant effect were also observed.In addition,the expression levels of key genes and protein(EGFR,TIMP-1,COL1A2,COL3A1,TGF-βⅠ,TGF-βⅡ)were increased,while those of MMP-1,MMP-3 in the skin of mouse were decreased in the RHC intervention groups.In conclusion,RHC has a good anti-aging effect on the skin,and its mechanism may be related to increase antioxidant effect,promote the EGFR,TGF pathway,and modulate the expression balance of MMPs and TIMPs,promote the proliferation and differentiation of epidermal cells,and the synthesis of collagen.

关 键 词：重组人源胶原蛋白 D-半乳糖 衰老模型 抗衰老 脂褐素 

分 类 号：TS201.4[轻工技术与工程—食品科学]