Formulation Containing Phytosomes of Carotenoids from Nyctanthes arbor-tristis and Tagetes patula Protect D-galactose Induced Skin Aging in Mice  

作　　者：Archana Arvind Naik Chhaya Harihar Gadgoli Arvind Bhimrao Naik 

机构地区：[1]Saraswathi Vidya Bhavan’s College of Pharmacy,Kalyan Shil Road,Sonarpada,Dombivli(E),Maharashtra 421204,India [2]Gahlot Institute of Pharmacy,Plot No 59,Sector 14,Koparkhairane,Navi Mumbai,Maharashtra 400709,India

出　　处：《Clinical Complementary Medicine and Pharmacology》2023年第1期35-46,共12页临床补充医学和药理学（英文）

基　　金：supported by National Medicinal Plant Board(NMPB),Dept.of Ayush,Government of India.(Project No.R&D/MS 04/2010).

摘　　要：Background:Carotenoids play important role in delay of aging process.Orange coloured tubular calyx of flowers of Nyctanthes arbor-tristis contains crocin,an apocarotenoid which forms a major component of stigma of saffron.Due to presence of crocin in orange coloured tubular calyx of Nyctanthes arbor-tristis,it can be used as an economical substitute to saffron for its medicinal and cosmetic utilities.Lutein from flower petals of Tagetes patula L.,is another popular carotenoid which has antioxidant effect and many health benefits.The carotenoids are highly unstable when exposed to atmosphere.One of the ways to improve stability of these phytoconstituents,is their entrapment in phytosomes.Preparation of phytosomes will have dual advantages of improving stability as well as bioavailability of molecule.Objective:In the present study crocin and lutein rich extracts were entrapped into phytosomes to improve stability and efficacy of topical preparation.Methods:The phytosomes of Carotenoid rich extract of tubular calyx of Nyctanthes arbor-tristis L.and the petals of Tagetes patula L.(standardized for crocin and lutein content)were prepared using lipid film hydration technique and these phytosomes were then incorporated into gel base.The gel formulation was evaluated for stability as per ICH guidelines.Efficacy of formulation was evaluated by d-galactose induced aging model.Aging in skin was induced by administration of d-galactose(100 mg/kg bwsc.)to albino mice for 42 days.The gel formulation was applied topically for 42 days.Then the effect of formulation on skin aging was evaluated by estimation of biochemical parameters viz.glutathione and malondialdehyde(MDA)and histopathological studies of treated skin samples.Expression of COL type I and elastin genes in skin samples was also carried out by RT-PCR.Results:Percent entrapment(%w/w)of crocin and lutein in phytosomes were found to be 60.20%and 50.81%,respectively.Accelerated stability studies showed improvement in stability of carotenoids viz.crocin and lutein and the

关 键 词：AGING CROCIN Phytosomes Nyctanthes arbor-tristis Collagen Elastin Galactose induced aging 

分 类 号：R285.5[医药卫生—中药学]