Ameliorative effects of glycine on cobalt chloride-induced hepato-renal toxicity in rats  

作　　者：Oluwafikemi Temitayo Iji Temitayo Olabisi Ajibade Oluwaseun Olanrewaju Esan Omolola Victoria Awoyomi Ademola Adetokunbo Oyagbemi Moses Olusola Adetona Temidayo Olutayo Omobowale Momoh Audu Yakubu Oluwafemi Omoniyi Oguntibeju Evaristus Nwulia 

机构地区：[1]Federal College of Animal Health and Production Technology,Moor Plantation,Ibadan,Nigeria [2]Department of Veterinary Physiology and Biochemistry,Faculty of Veterinary Medicine,University of Ibadan,Ibadan,Nigeria [3]Department of Veterinary Medicine,Faculty of Veterinary Medicine,University of Ibadan,Ibadan,Nigeria [4]Department of Anatomy,Faculty of Basic Medical Sciences,University of Ibadan,Ibadan,Nigeria [5]Department of Environmental and Interdisciplinary Sciences,College of Science,Engineering&Technology,COPHS,Texas Southern University,Houston,Texas,USA [6]Department of Biomedical Sciences,Faculty of Health and Wellness Sciences,Cape Peninsula University of Technology,Bellville,South Africa [7]Department of Psychiatry and Behavioral Sciences,Howard University Hospital,College of Medicine,Howard University,Washington,District of Columbia,USA

出　　处：《Animal Models and Experimental Medicine》2023年第2期168-177,共10页动物模型与实验医学（英文）

摘　　要：Background:The important roles of liver and kidney in the elimination of injurious chemicals make them highly susceptible to the noxious activities of various toxicants including cobalt chloride(CoCl_(2)).This study was designed to investigate the role of glycine in the mitigation of hepato-renal toxicities associated with CoCl_(2)exposure.Methods:Forty-two(42)male rats were grouped as Control;(CoCl_(2);300 ppm);CoCl_(2)+Glycine(50 mg/kg);CoCl_(2)+Glycine(100 mg/kg);Glycine(50 mg/kg);and Glycine(100 mg/kg).The markers of hepatic and renal damage,oxidative stress,the antioxidant defense system,histopathology,and immunohistochemical localization of neutrophil gelatinase associated lipocalin(NGAL)and renal podocin were evaluated.Results:Glycine significantly reduced the markers of oxidative stress(malondialdehyde content and H_(2)O_(2) generation),liver function tests(ALT,AST,and ALP),markers of renal function(creatinine and BUN),and decreased the expression of neutrophil gelatinase-associated lipocalin(NGAL)and podocin compared with rats exposed to CoCl_(2)toxicity without glycine treatment.Histopathology lesions including patchy tubular epithelial necrosis,tubular epithelial degeneration and periglomerular inflammation in renal tissues,and severe portal hepatocellular necrosis,inflammation,and duct hyperplasia were observed in hepatic tissues of rats exposed to CoCl_(2)toxicity,but were mild to absent in glycine-treated rats.Conclusion:The results of this study clearly demonstrate protective effects of glycine against CoCl_(2)-induced tissue injuries and derangement of physiological activities of the hepatic and renal systems in rats.The protective effects are mediated via augmentation of total antioxidant capacity and upregulation of NGAL and podocin expression.

关 键 词：cobalt chloride HEPATOTOXICITY NEPHROTOXICITY oxidative stress PODOCIN 

分 类 号：R692[医药卫生—泌尿科学]