孜然枯茗醛对实验性胃溃疡大鼠胃黏膜的保护作用  

The protective effect of cuminaldehyde on gastric mucosa in Rattus norregicus of experimental gastric ulcer

作　　者：周慧英 邓红雨 康瑞萍 王卓 艾菲热.阿布都艾尼 黄罗冬 索菲娅[1] ZHOU Hui-ying;DENG Hong-yu;KANG Rui-ping;WANG Zhuo;AIFEIRE Abuduaini;Huang Luo-dong;SUO Fei-ya(College of Life Science and Technology,Xinjiang University,Urumchi 830046,China;College of Life Science and Technology,Guangxi University,Nanning 530004,China;Tacheng Normal School,Tacheng,Xinjiang 834700,China)

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830046 [2]广西大学生命科学与技术学院,广西南宁530004 [3]塔城地区师范学校,新疆塔城834700

出　　处：《中国药理学通报》2023年第5期946-952,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 31860077)。

摘　　要：目的采用乙醇损伤构建大鼠的胃溃疡模型,探究孜然果实中枯茗醛(cuminaldehyde,CUM)对胃溃疡的作用与保护机制。方法选择36只雄性的SD大鼠,分为6组:对照组、模型组、奥美拉唑阳性对照组以及CUM低、中、高剂量组,每组6只。连续灌胃7 d后,采用无水乙醇法对大鼠进行急性胃溃疡实验。检测各组大鼠的胃溃疡面积、抑制率、胃组织抗氧化能力、血清炎症因子与胃黏膜保护因子的表达。结果CUM组可明显降低胃黏膜溃疡面积,提高胃溃疡抑制率,CUM高剂量组抑制率最高可达74.65%,此外,CUM 4的大鼠胃组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH)的活性明显升高,胃黏膜保护因子血清前列腺素E_(2)(PGE_(2))和胃组织NO的含量也明显增加,降低了丙二醛(MDA)的含量。与模型组比较,CUM降低了大鼠血清中肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)的含量,髓过氧化物酶(MPO)的活性明显降低。结论CUM具有良好的抗氧化应激能力和抗炎活性,可提高保护因子的表达,增强胃黏膜的防御能力,对大鼠急性胃溃疡产生明显的保护作用。Aim To explore the effect of cuminaldehyde in cumin fruit on gastric ulcer and the protective mechanism via establishing the gastric ulcer model of rats was by ethanol injury.Methods Thirty-six male R.norregicus were divided into six groups:control group,model group,omeprazole positive control group and cuminaldehyde low,medium and high dosage groups.After seven days of continuous intragastric administration,the acute gastric ulcer of R.norregicus was tested by absolute alcohol.Gastric ulcer area,inhibition rate,gastric tissue antioxidant activity,serum inflammatory factors and gastric mucosal protective factors were detected in different groups.Results The results showed that cuminaldehyde significantly reduced the area of gastric ulcer and increased the inhibition rate of gastric ulcer.The inhibition rate of cuminaldehyde at high dose group was up to 74.65%,the activities of superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GSH)in gastric tissue significantly increased,and the contents of serum prolandin E_(2)(PGE_(2))and NO in gastric tissue also significantly increased.The content of malondialdehyde(MDA)decreased.Compared with the model group,cuminaldehyde decreased the content of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)in rat serum,and decreased the activity of myeloperoxidase(MPO).Conclusions Cuminaldehyde has good antioxidant stress ability and anti-inflammatory activity,increases the expression of protective factors,enhances the defense ability of gastric mucosa,and has obvious protective effect on acute gastric ulcer in R.norregicus.

关 键 词：孜然 枯茗醛 胃溃疡 溃疡指数 抗氧化 炎症因子 

分 类 号：R-332[医药卫生] R284.1R322.44R364.5R573.1