牡丹籽壳中黄酮类化合物提取及组分分析  

Extraction and composition analysis of flavonoids in peony seed shells

作　　者：邹平[1] 张迎阳[1] 邹林玲 ZOU Ping;ZHANG Ying-yang;ZOU Lin-ling(School of Food,Changzhou University,Changzhou,Jiangsu 213164,China)

机构地区：[1]常州大学食品学院,江苏常州213164

出　　处：《食品与机械》2023年第4期44-50,共7页Food and Machinery

基　　金：江苏省科技支撑计划(编号:BE2018316)。

摘　　要：目的:探究牡丹籽壳中天然黄酮化合物(MDZK)的抗氧化性。方法:采用溶剂回流提取法对牡丹籽壳总黄酮进行提取,采用紫外—可见分光光度计法测定MDZK含量和抗氧化性能,并采用LC-MS法对提取的总黄酮进行组分分析。结果:当提取溶剂为70%乙醇,提取温度为70℃,料液比为1∶25(g/mL),提取时间60 min时,MDZK得率最高为(10.54±0.13)%;当MDZK质量浓度为120μg/mL时,其对DPPH自由基、超氧阴离子自由基和OH自由基的最高清除率分别为58.54%,55.72%,49.07%,表明MDZK具有一定的抗氧化作用且效果良好;通过与数据库比对鉴定出20多种黄酮化合物及其衍生物,其中含量最多的为木犀草苷、圣草酚、山奈酚、芹菜素和新橙皮苷等。结论:牡丹籽壳中的黄酮化合物抗氧化活性良好,可作为抗氧化产品用于食品工业或医药等领域。Objective:To extract natural flavonoid compounds(MDZK)from peony seed husks for composition analysis and antioxidant research.Methods:The total flavonoids of peony seed shell were extracted by solvent reflux extraction method.The ultraviolet-visible spectrophotometer method was used to determine the MDZK content and antioxidant properties;The total flavonoids extracted were analyzed by LC-MS method.Results:When the solvent was 70%ethanol,the extraction temperature was 70℃,the feed-to-liquid ratio was 1∶25(g/mL),and the extraction time was 60 min,the MDZK yield was the highest,which was(10.54±0.13)%.When the concentration of MDZK reached 120μg/mL,the highest clearance rates of DPPH radicals,O-2 radicals and·OH radicals were 58.54%,55.72%and 49.07%,respectively,which showed that MDZK had certain antioxidant effects and good effects.More than 20 flavonoid compounds and their derivatives were identified by comparison with the database,of which the most abundant content were Luteoloside,Eriodictyol Kaempfero,Apigenin and Neohesperidin.Conclusion:The flavonoid compounds in the shell of peony seeds have good antioxidant activity and can be used as antioxidant products in food industry or medicine,and have wide application prospects.

关 键 词：牡丹籽壳 黄酮类 抗氧化活性 液质联用 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程]