Amygdalin Ameliorates Liver Fibrosis through Inhibiting Activation of TGF-β/Smad Signaling  

作　　者：XIAO Zhun JI Qiang FU Ya-dong GAO Si-qi HU Yong-hong LIU Wei CHEN Gao-feng MU Yong-ping CHEN Jia-mei LIU Ping 

机构地区：[1]Institute of Liver Diseases,Key Laboratory of Liver and Kidney Diseases(Ministry of Education),Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China [2]E-Institute of Shanghai Municipal Education Commission Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China [3]Shanghai Key Laboratory of Traditional Chinese Medicine,Shanghai 201203,China

出　　处：《Chinese Journal of Integrative Medicine》2023年第4期316-324,共9页中国结合医学杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China(Nos.81530101,81973613 and 81603681);Shanghai Rising-Star Program(No.19QA1408900)。

摘　　要：Objective:To observe the effect of amygdalin on liver fibrosis in a liver fibrosis mouse model,and the underlying mechanisms were partly dissected in vivo and in vitro.Methods:Thirty-two male mice were randomly divided into 4 groups,including control,model,low-and high-dose amygdalin-treated groups,8 mice in each group.Except the control group,mice in the other groups were injected intraperitoneally with 10%carbon tetrachloride(CCl4)-olive oil solution 3 times a week for 6 weeks to induce liver fibrosis.At the first 3 weeks,amygdalin(1.35 and 2.7 mg/kg body weight)were administered by gavage once a day.Mice in the control group received equal quantities of subcutaneous olive oil and intragastric water from the fourth week.At the end of 6 weeks,liver tissue samples were harvested to detect the content of hydroxyproline(Hyp).Hematoxylin and eosin and Sirius red staining were used to observe the inflammation and fibrosis of liver tissue.The expressions of collagenⅠ(Col-Ⅰ),alpha-smooth muscle actin(α-SMA),CD31 and transforming growth factorβ(TGF-β)/Smad signaling pathway were detected by immunohistochemistry,quantitative real-time polymerase chain reaction and Western blot,respectively.The activation models of hepatic stellate cells,JS-1and LX-2 cells induced by TGF-β1 were used in vitro with or without different concentrations of amygdalin(0.1,1,10μmol/L).The effect of different concentrations of amygdalin on the expressions of liver sinusoidal endothelial cells(LSECs)dedifferentiation markers CD31 and CD44 were observed.Results:High-dose of amygdalin significantly reduced the Hyp content and percentage of collagen positive area,and decreased the mRNA and protein expressions of Col-Ι,α-SMA,CD31 and p-Smad2/3 in liver tissues of mice compared to the model group(P<0.01).Amygdalin down-regulated the expressions of Col-Ⅰandα-SMA in JS-1 and LX-2 cells,and TGFβR1,TGFβR2 and p-Smad2/3 in LX-2 cells compared to the model group(P<0.05 or P<0.01).Moreover,1 and 10μmol/L amygdalin inhibited the mRNA and prote

关 键 词：AMYGDALIN liver fibrosis hepatic stellate cells liver sinusoidal endothelial cells TGF-β/Smad signalingpathway 

分 类 号：R575.2[医药卫生—消化系统]